Team:Calgary/29 September 2009
From 2009.igem.org
(Difference between revisions)
Prima.moinul (Talk | contribs) |
Prima.moinul (Talk | contribs) |
||
Line 405: | Line 405: | ||
This image illustrates the results of colony PCR of rbs-aiiA-B0015 | This image illustrates the results of colony PCR of rbs-aiiA-B0015 | ||
The initial denaturation process was set to 94oC for 6 mins, followed by 30 seconds of annealing time at 94oC and 45 seconds extension time for 55oC. | The initial denaturation process was set to 94oC for 6 mins, followed by 30 seconds of annealing time at 94oC and 45 seconds extension time for 55oC. | ||
+ | <br> | ||
[[Image:2009igem.calgary.colonypcr.png|700px]] | [[Image:2009igem.calgary.colonypcr.png|700px]] | ||
+ | <br> | ||
<br> | <br> | ||
For this PCR, BBK-CP-R and B0034-F primers were used. Wells 1,2,3,4,5 and 6 represent colonies 1,2,3,4,5,and negative control. The expected size of the construct is ~1.1kb | For this PCR, BBK-CP-R and B0034-F primers were used. Wells 1,2,3,4,5 and 6 represent colonies 1,2,3,4,5,and negative control. The expected size of the construct is ~1.1kb |
Latest revision as of 03:23, 22 October 2009
UNIVERSITY OF CALGARY