Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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== October 7, 2009 == | == October 7, 2009 == | ||
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+ | m stands for multipromoter !! | ||
Cultures were prepared as follows: | Cultures were prepared as follows: | ||
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10 µL IPTG 1M + - + | 10 µL IPTG 1M + - + | ||
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For the induction of protein production we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | For the induction of protein production we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four |
Revision as of 03:37, 22 October 2009