Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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Again an Agarose was run 1hr. at 85 V. to se the relative concentration of each sample that is going to be use | Again an Agarose was run 1hr. at 85 V. to se the relative concentration of each sample that is going to be use | ||
for the ligation reaction. | for the ligation reaction. | ||
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Ligation Reaction: | Ligation Reaction: | ||
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== October 7, 2009 == | == October 7, 2009 == | ||
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+ | REGISTRY SITE FOR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K242101 MULTIPROMOTER] BBa_K242101 | ||
+ | |||
+ | m stands for multipromoter !! | ||
Cultures were prepared as follows: | Cultures were prepared as follows: | ||
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100 µL Cam 20 µg/µL + + + | 100 µL Cam 20 µg/µL + + + | ||
10 µL IPTG 1M + - + | 10 µL IPTG 1M + - + | ||
+ | |||
For the induction of protein production we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | For the induction of protein production we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | ||
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Results: | Results: | ||
- | Bl21/m+ Bl21/m+ Bl21/m | + | Bl21/m+ Bl21/m+ Bl21/m- |
IPTG + - + | IPTG + - + | ||
++++ ++ - Fluorescence | ++++ ++ - Fluorescence | ||
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This results support the functioning of multipromoter for T7 polymerase. | This results support the functioning of multipromoter for T7 polymerase. | ||
- | Great NEWS!!! our promoter worked for T7 polymerase | + | Great NEWS!!! our promoter worked for T7 RNA polymerase |
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+ | For future experiments to get a better characterization look result site. | ||
== October 20, 2009 == | == October 20, 2009 == | ||
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== October 21, 2009 == | == October 21, 2009 == | ||
+ | Preparation of parts for sending them to the registry!!! | ||
+ | =References= | ||
+ | To see all the literature that we used to develop the construction of control system pleas visit | ||
+ | references site and look for ogr, cox, P2 and P4 papers [https://2009.igem.org/Team:LCG-UNAM-Mexico/Description#References References]. | ||
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Latest revision as of 03:48, 22 October 2009