Team:LCG-UNAM-Mexico:Journals:Uriel
From 2009.igem.org
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== October 7, 2009 == | == October 7, 2009 == | ||
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+ | REGISTRY SITE FOR [http://partsregistry.org/wiki/index.php?title=Part:BBa_K242101 MULTIPROMOTER] BBa_K242101 | ||
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+ | m stands for multipromoter !! | ||
Cultures were prepared as follows: | Cultures were prepared as follows: | ||
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10 µL IPTG 1M + - + | 10 µL IPTG 1M + - + | ||
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For the induction of protein production we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | For the induction of protein production we started our culture at .1 abs 620nm in 100mL LB and waited until it reached 0.35 abs 620 nm then we added IPTG to a final concentration of 0.1mM and then we leave the culture media for four | ||
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== October 21, 2009 == | == October 21, 2009 == | ||
+ | Preparation of parts for sending them to the registry!!! | ||
+ | =References= | ||
+ | To see all the literature that we used to develop the construction of control system pleas visit | ||
+ | references site and look for ogr, cox, P2 and P4 papers [https://2009.igem.org/Team:LCG-UNAM-Mexico/Description#References References]. | ||
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Latest revision as of 03:48, 22 October 2009