Team:Lethbridge

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Latest revision as of 03:53, 22 October 2009

Project Overview

The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy. Microbial fuel cells (MFCs) use biological systems to produce an electrical current. Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).

We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery. The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids. As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer. Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.

References

Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic bioelectrochemical cell utilizing cyanobacteria and water-generating oxidase. Enzyme and Microbial Technology. 29, 225-231

University of Lethbridge Sponsors

University of Lethbridge Supporters

The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support. Including, but not limited to:

The Wieden Lab

The Kothe Lab

The Selinger Lab

The Biochemistry Teaching Lab and coordinators

The University of Lethbridge Student's Union

David Franz

Nathan Puhl

John Thibault

Sebastian Machula

and

Tony Russell

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+</head>
-<!-- QuickMenu Structure [Menu 0] -->
+<body>
 <ul id="qm0" class="qmmc">
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge">Home</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge" style="width:20px;">Home</a>
-		<ul style="width:92px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery">Project Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery" style="width:90px;">Project Overview</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge#University_of_Lethbridge_Sponsors">Our Sponsors</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Who We Are</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Team" style="width:3px;">Team</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project">The Project</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project" style="width:10px;">Project</a>
-		<ul style="width:138px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Overview">Overview </a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Project_Details">Project Details</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Experiments">Experiments</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#The_Experiments">Experiments</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Results">Results</a></li>
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics">Ethics</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics" style="width:40px;">Ethics and Safety</a>
-		<ul style="width:94px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Overview">Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Ethics_and_SynBio" style="width:80px;">Ethics and Synbio</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#The_Survey">The Survey</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Results">Results</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Safety">Safety</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Analysis">Analysis</a></li>
+                </ul></li>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook" style="width:3px;">Notebook</a>
+		<ul>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#Calendar">Calendar</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#May">May</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#June">June</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#July">July</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#August">August</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#September">September</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#October">October</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook">Notebook</a></li>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings" style="width:10px;">Meetings</a>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings">Team Meetings</a>
-		<ul style="width:164px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#April">April</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#May">May</a></li>
@@ Line 250: / Line 272: @@
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#September">September</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#October">October</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#Novermber">November</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#Events">Events</a></li>
-		</ul></li>
+                </ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling">Modeling</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling" style="width:10px;">Modeling</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts">Parts Submitted to the Registry</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts" style="width:10px;">Parts</a>
-		<ul style="width:292px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_1">Part 1</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Favourites">Favourites</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_2">Part 2</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Individual_Parts">Individual Parts</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_3">Part 3</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Constructs.2FDevices">Constructs</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Part_4">Part 4</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediates</a></li>
 		</ul></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Collaboration" style="width:25px;">Collaboration</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:117px;">Extra Information on SynBio</a></li>
 <li class="qmclear">&nbsp;</li></ul>
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+<script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 </body>
 </html>
-<br />
+[[Image:Igembanner.jpg|965px|]]
-A University team from Lethbridge, Alberta, Canada
+==Project Overview==
-<!--|[[Image:Example_logo.png|200px|right|frame]]-->
-{|align="justify"
+[[Image:Biobattery.png|right|275px]]
-|-
-|
-''
-== Our Project: The BioBattery ==
+The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy.  Microbial fuel cells (MFCs) use biological systems to produce an electrical current.  Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).
-Our BioBattery, made from photosynthetic bacteria will be able to produce energy and its own food by converting carbon dioxide into water, oxygen and sugars, making it an indefinitely running sustainable energy source.  Its ability|[[Image:Group_Photo_1.png|200px|frame]] to use atmospheric CO2 means it will be able to create energy while REMOVING it from the atmosphere, making it a carbon negative fuel source, something that is going to be necessary to solve the world's energy crisis.  Because our BioBattery will be made from a cyanobacteria, it will also be able to fix Nitrogen, the process which is necessary to build amino acids and something humans cannot do on their own.  These abilities, fixing carbon dioxide and nitrogen may even make the BioBattery useful for terraforming.
-''
-== University of Lethbridge Sponsors ==
+We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery.  The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids.   As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer.  Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.
+'''References'''
+Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic
+bioelectrochemical cell utilizing cyanobacteria and water-generating
+oxidase. Enzyme and Microbial Technology. 29, 225-231
+==University of Lethbridge Sponsors==
 <html>
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+<html>
+<body>
+<table width="965" border="0.5">
+ <tr>
+<td>
 <a href="http://www.uleth.ca" target="_blank">
-<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_fiat_143.jpg"></a>
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_fiat_143.jpg"></a></td>
+<td>
+<a href="http://www.albertaingenuity.ca/international/genetically/engineered/machines/igem" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/AlbertaIngenuitylogo.jpg"></a></td>
+<td><a href="http://www.rainin.com/canada/default_can.asp" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/RAININ_Logo_Japan_big.jpg"></a>
+</td>
+</tr>
+<tr>
+<td><a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_mrgene.jpg"></a></td>
+<td>
+<a href="http://www.viterra.ca/portal/wps/portal/viterra" target="_blank">
+<img src="http://s904.photobucket.com/albums/ac246/MegLand/Viterra.jpg"></a>
+</td>
+<td>
+<a href="http://www.vwrcanlab.com/" target="_blank">
+<img src="http://s904.photobucket.com/albums/ac246/MegLand/VWR.jpg"></a></td>
+</tr>
+</table>
-<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
+</body>
-<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_mrgene.jpg"></a>
+</html>
-<a href="http://www.albertaingenuity.ca/international/genetically/engineered/machines/igem" target="_blank">
-<img src="http://i770.photobucket.com/albums/xx343/ulethigem/AlbertaIngenuity.gif"></a>
+<html>
+<head>
+<html>
+<head>
+<!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
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+/*!!!!!!!!!!! QuickMenu Core CSS [Do Not Modify!] !!!!!!!!!!!!!*/
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+</head>
+<body>
+<ul id="qm0" class="qmmc">
+	<li><a href="https://2009.igem.org/Team:Lethbridge">Home</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge">Top of Page</a></li>
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 </html>
+==University of Lethbridge Supporters==
+The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support.  Including, but not limited to:
+The Wieden Lab
+The Kothe Lab
+The Selinger Lab
+The Biochemistry Teaching Lab and coordinators
+The University of Lethbridge Student's Union
+David Franz
+Nathan Puhl
+John Thibault
+Sebastian Machula
+and
+Tony Russell