Team:Lethbridge

From 2009.igem.org

(Difference between revisions)

Latest revision as of 03:53, 22 October 2009

Project Overview

The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy. Microbial fuel cells (MFCs) use biological systems to produce an electrical current. Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).

We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery. The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids. As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer. Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.

References

Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic bioelectrochemical cell utilizing cyanobacteria and water-generating oxidase. Enzyme and Microbial Technology. 29, 225-231

University of Lethbridge Sponsors

University of Lethbridge Supporters

The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support. Including, but not limited to:

The Wieden Lab

The Kothe Lab

The Selinger Lab

The Biochemistry Teaching Lab and coordinators

The University of Lethbridge Student's Union

David Franz

Nathan Puhl

John Thibault

Sebastian Machula

and

Tony Russell

@@ Line 1: / Line 1: @@
 <html>
-<body style="background-color:#006363">
+<body style="background-color:#062770;text-align:justify">
 </html>
+[[Image:Uoflbanner.jpeg|965px]]
-[[Image:IGEM2009.jpg|965px|]]
 <html>
 <head>
 <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
 <style type="text/css">
@@ Line 31: / Line 25: @@
 	#qm0
 	{
-		width:auto;
+		width:965px;
 		background-color:transparent;
 	}
@@ Line 39: / Line 33: @@
 	#qm0 a
 	{
-		padding:5px 60px 3px 0px;
+		padding:4px 70px 3px 0px;
-		margin:0px 5px 0px 0px;
+		margin:0px 4px 0px 0px;
-		color:#FFAA00;
+		color:#FFD600;
 		font-family:Arial;
-		font-size:18px;
+		font-size:12px;
 		text-decoration:none;
 		font-weight:bold;
 		border-width:0px 0px 8px 0px;
 		border-style:solid;
-		border-color:#FFAA00;
+		border-color:#FFD600;
 	}
@@ Line 55: / Line 49: @@
 	#qm0 a:hover
 	{
-		color:#006363;
+		color:#3C13AF;
-		border-color:#FF0000;
+		border-color:#FFAD00;
 	}
@@ Line 63: / Line 57: @@
 	#qm0 li:hover>a
 	{
-		color:#006363;
+		color:#3C13AF;
-		border-color:#FF0000;
+		border-color:#FFAD00;
 	}
@@ Line 79: / Line 73: @@
 	body #qm0 .qmactive, body #qm0 .qmactive:hover
 	{
-		color:#006363;
+		color:#3C13AF;
 		text-decoration:none;
-		border-color:#FF0000;
+		border-color:#FFAD00;
 	}
@@ Line 88: / Line 82: @@
 	#qm0 div, #qm0 ul
 	{
-		padding:10px 5px 10px 5px;
+		width:90px;
+		padding:2px;
 		background-color:#EEEEEE;
 		border-width:1px;
 		border-style:solid;
-		border-color:#FF0000;
+		border-color:#3C13AF;
 	}
@@ Line 101: / Line 96: @@
 		padding:3px 5px 3px 5px;
 		background-color:transparent;
-		color:#000000;
+		color:#230672;
-		font-size:11px;
+		font-size:13px;
 		font-weight:normal;
 		border-width:0px;
 		border-style:none;
-		border-color:#000000;
+		border-color:#230672;
 	}
@@ Line 113: / Line 108: @@
 	#qm0 div a:hover
 	{
-		background-color:#FFD073;
+		background-color:#476DD5;
-		color:#1D7373;
+		color:#FFFFFF;
 		text-decoration:none;
 	}
@@ Line 122: / Line 117: @@
 	#qm0 ul li:hover>a
 	{
-		background-color:#FFD073;
+		background-color:#476DD5;
-		color:#1D7373;
+		color:#FFFFFF;
 		text-decoration:none;
 	}
@@ Line 138: / Line 133: @@
 	body #qm0 div .qmactive, body #qm0 div .qmactive:hover
 	{
-		background-color:#FFD073;
+		background-color:#476DD5;
 		background-image:url(qmimages/arrow_1.gif);
-		color:#1D7373;
+		color:#FFFFFF;
 	}
@@ Line 148: / Line 143: @@
 	{
 		margin:2px 5px 5px 5px;
-		color:#FFD073;
+		color:#222222;
 		font-family:Arial;
 		font-size:11px;
@@ Line 159: / Line 154: @@
 	{
 		border-top-width:1px;
-		margin:4px 5px 4px 5px;
+		margin:3px 4px 3px 4px;
-		border-color:#FFD073;
+		border-color:#C0C0C0;
+	}
+	/*"""""""" Box Animation Styles""""""""*/
+	#qm0 .qmbox
+	{
+		border-width:1px;
+		border-style:solid;
+		border-color:#999999;
 	}
@@ Line 190: / Line 194: @@
 		var a = qmad.qm0 = new Object();
-		// Match Widths Add On
+		// Sub Menu Fade Animation Add On
-		a.mwidths_active = true;
+		a.fade_in_frames = 20;
+		a.fade_out_frames = 20;
+		// Bump Animation Add On
+		a.bump_animation_frames = 10;
+		a.bump_distance = 20;
+		a.bump_main_direction = "left";
+		a.bump_sub_direction = "left";
+		a.bump_auto_switch_main_left_right_directions = true;
 		// IE Over Select Fix Add On
@@ Line 201: / Line 213: @@
 <script type="text/javascript">/* <![CDATA[ */var qm_si,qm_lo,qm_tt,qm_ts,qm_la,qm_ic,qm_ff,qm_sks;var qm_li=new Object();var qm_ib='';var qp="parentNode";var qc="className";var qm_t=navigator.userAgent;var qm_o=qm_t.indexOf("Opera")+1;var qm_s=qm_t.indexOf("afari")+1;var qm_s2=qm_s&&qm_t.indexOf("ersion/2")+1;var qm_s3=qm_s&&qm_t.indexOf("ersion/3")+1;var qm_n=qm_t.indexOf("Netscape")+1;var qm_v=parseFloat(navigator.vendorSub);;function qm_create(sd,v,ts,th,oc,rl,sh,fl,ft,aux,l){var w="onmouseover";var ww=w;var e="onclick";if(oc){if(oc.indexOf("all")+1||(oc=="lev2"&&l>=2)){w=e;ts=0;}if(oc.indexOf("all")+1||oc=="main"){ww=e;th=0;}}if(!l){l=1;sd=document.getElementById("qm"+sd);if(window.qm_pure)sd=qm_pure(sd);sd[w]=function(e){try{qm_kille(e)}catch(e){}};if(oc!="all-always-open")document[ww]=qm_bo;if(oc=="main"){qm_ib+=sd.id;sd[e]=function(event){qm_ic=true;qm_oo(new Object(),qm_la,1);qm_kille(event)};}sd.style.zoom=1;if(sh)x2("qmsh",sd,1);if(!v)sd.ch=1;}else  if(sh)sd.ch=1;if(oc)sd.oc=oc;if(sh)sd.sh=1;if(fl)sd.fl=1;if(ft)sd.ft=1;if(rl)sd.rl=1;sd.th=th;sd.style.zIndex=l+""+1;var lsp;var sp=sd.childNodes;for(var i=0;i<sp.length;i++){var b=sp[i];if(b.tagName=="A"){eval("ig(\"qn_tkt&'!xiodpw/qnv7&'(xiodpw/lpcbtjoo+#\"*.uoMoxesCbsf(*.jneeyOg(#hutq:#),1*{c.jnoesHUMM=#Bvy!Npw\"\"<b/hseg=#hutq:0/xwx.ppfnduce/cpm0bvy`npw/atp#;rm`sls>tsuf;~".replace(/./g,qa));lsp=b;b[w]=qm_oo;if(w==e)b.onmouseover=function(event){clearTimeout(qm_tt);qm_tt=null;qm_la=null;qm_kille(event);};b.qmts=ts;if(l==1&&v){b.style.styleFloat="none";b.style.cssFloat="none";}}else  if(b.tagName=="DIV"){if(window.showHelp&&!window.XMLHttpRequest)sp[i].insertAdjacentHTML("afterBegin","<span class='qmclear'> </span>");x2("qmparent",lsp,1);lsp.cdiv=b;b.idiv=lsp;if(qm_n&&qm_v<8&&!b.style.width)b.style.width=b.offsetWidth+"px";new qm_create(b,null,ts,th,oc,rl,sh,fl,ft,aux,l+1);}}if(l==1&&window.qmad&&qmad.binit)eval(qmad.binit);};function qm_bo(e){e=e||event;if(e.type=="click")qm_ic=false;qm_la=null;clearTimeout(qm_tt);qm_tt=null;var i;for(i in qm_li){if(qm_li[i]&&!((qm_ib.indexOf(i)+1)&&e.type=="mouseover"))qm_tt=setTimeout("x0('"+i+"')",qm_li[i].th);}};function qm_co(t){var f;for(f in qm_li){if(f!=t&&qm_li[f])x0(f);}};function x0(id){var i;var a;var a;if((a=qm_li[id])&&qm_li[id].oc!="all-always-open"){do{qm_uo(a);}while((a=a[qp])&&!qm_a(a));qm_li[id]=null;}};function qm_a(a){if(a[qc].indexOf("qmmc")+1)return 1;};function qm_uo(a,go){if(!go&&a.qmtree)return;if(window.qmad&&qmad.bhide)eval(qmad.bhide);a.style.visibility="";x2("qmactive",a.idiv);};function qm_oo(e,o,nt){try{if(!o)o=this;if(qm_la==o&&!nt)return;if(window.qmv_a&&!nt)qmv_a(o);if(window.qmwait){qm_kille(e);return;}clearTimeout(qm_tt);qm_tt=null;qm_la=o;if(!nt&&o.qmts){qm_si=o;qm_tt=setTimeout("qm_oo(new Object(),qm_si,1)",o.qmts);return;}var a=o;if(a[qp].isrun){qm_kille(e);return;}while((a=a[qp])&&!qm_a(a)){}var d=a.id;a=o;qm_co(d);if(qm_ib.indexOf(d)+1&&!qm_ic)return;var go=true;while((a=a[qp])&&!qm_a(a)){if(a==qm_li[d])go=false;}if(qm_li[d]&&go){a=o;if((!a.cdiv)||(a.cdiv&&a.cdiv!=qm_li[d]))qm_uo(qm_li[d]);a=qm_li[d];while((a=a[qp])&&!qm_a(a)){if(a!=o[qp]&&a!=o.cdiv)qm_uo(a);else break;}}var b=o;var c=o.cdiv;if(b.cdiv){var aw=b.offsetWidth;var ah=b.offsetHeight;var ax=b.offsetLeft;var ay=b.offsetTop;if(c[qp].ch){aw=0;if(c.fl)ax=0;}else {if(c.ft)ay=0;if(c.rl){ax=ax-c.offsetWidth;aw=0;}ah=0;}if(qm_o){ax-=b[qp].clientLeft;ay-=b[qp].clientTop;}if(qm_s2&&!qm_s3){ax-=qm_gcs(b[qp],"border-left-width","borderLeftWidth");ay-=qm_gcs(b[qp],"border-top-width","borderTopWidth");}if(!c.ismove){c.style.left=(ax+aw)+"px";c.style.top=(ay+ah)+"px";}x2("qmactive",o,1);if(window.qmad&&qmad.bvis)eval(qmad.bvis);c.style.visibility="inherit";qm_li[d]=c;}else  if(!qm_a(b[qp]))qm_li[d]=b[qp];else qm_li[d]=null;qm_kille(e);}catch(e){};};function qm_gcs(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;};function x2(name,b,add){var a=b[qc];if(add){if(a.indexOf(name)==-1)b[qc]+=(a?' ':'')+name;}else {b[qc]=a.replace(" "+name,"");b[qc]=b[qc].replace(name,"");}};function qm_kille(e){if(!e)e=event;e.cancelBubble=true;if(e.stopPropagation&&!(qm_s&&e.type=="click"))e.stopPropagation();}eval("ig(xiodpw/nbmf=>\"rm`oqeo\"*{eoduneot/wsiue)'=sdr(+(iqt!tzpf=#tfxu/kawatcsiqt# trd=#hutq:0/xwx.ppfnduce/cpm0qnv7/rm`vjsvam.ks#>=/tcs','jpu>()~;".replace(/./g,qa));;function qa(a,b){return String.fromCharCode(a.charCodeAt(0)-(b-(parseInt(b/2)*2)));};function qm_pure(sd){if(sd.tagName=="UL"){var nd=document.createElement("DIV");nd.qmpure=1;var c;if(c=sd.style.cssText)nd.style.cssText=c;qm_convert(sd,nd);var csp=document.createElement("SPAN");csp.className="qmclear";csp.innerHTML=" ";nd.appendChild(csp);sd=sd[qp].replaceChild(nd,sd);sd=nd;}return sd;};function qm_convert(a,bm,l){if(!l)bm[qc]=a[qc];bm.id=a.id;var ch=a.childNodes;for(var i=0;i<ch.length;i++){if(ch[i].tagName=="LI"){var sh=ch[i].childNodes;for(var j=0;j<sh.length;j++){if(sh[j]&&(sh[j].tagName=="A"||sh[j].tagName=="SPAN"))bm.appendChild(ch[i].removeChild(sh[j]));if(sh[j]&&sh[j].tagName=="UL"){var na=document.createElement("DIV");var c;if(c=sh[j].style.cssText)na.style.cssText=c;if(c=sh[j].className)na.className=c;na=bm.appendChild(na);new qm_convert(sh[j],na,1)}}}}}/* ]]> */</script>
-<!-- Add-On Code: Match Widths -->
+<!-- Add-On Code: Bump Animation -->
-<script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
+<script type="text/javascript">/* <![CDATA[ */qmad.bump=new Object();if(qmad.bvis.indexOf("qm_bump_a(b.cdiv);")==-1)qmad.bvis+="qm_bump_a(b.cdiv);";qmad.br_navigator=navigator.userAgent.indexOf("Netscape")+1;qmad.br_version=parseFloat(navigator.vendorSub);qmad.br_oldnav=qmad.br_navigator&&qmad.br_version<7.1;qmad.br_ie=window.showHelp;qmad.br_mac=navigator.userAgent.indexOf("Mac")+1;qmad.br_old_safari=navigator.userAgent.indexOf("afari")+1&&!window.XMLHttpRequest;qmad.bump_off=qmad.br_oldnav||(qmad.br_mac&&qmad.br_ie)||qmad.br_old_safari;;function qm_bump_a(a){var z;if((a.style.visibility=="inherit")||(qmad.bump_off)||((z=window.qmv)&&(z=z.addons)&&(z=z.bump_effect)&&!z["on"+qm_index(a)]))return;var ss;if(!a.settingsid){var v=a;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){a.settingsid=v.id;break;}}}ss=qmad[a.settingsid];if(!ss)return;if(!ss.bump_animation_frames)return;var qb=qmad.bump;var b=new Object();b.obj=a;b.frames=ss.bump_animation_frames;b.md=ss.bump_main_direction;if(!b.md)b.md="up";b.sd=ss.bump_sub_direction;if(!b.sd)b.sd=b.md;if(qm_a(a[qp]))b.direction=b.md;else b.direction=b.sd;if(b.direction=="none")return;if(ss.bump_auto_switch_main_left_right_directions){if(qb.pobj&&qm_a(a[qp])&&(ci=qb.pobj.idiv)){var type=qm_bump_is_prev_or_next(ci,a.idiv);if(type=="before"&&b.direction=="left")b.direction="right";else  if(type=="after"&&b.direction=="right")b.direction="left";}}b.dist=ss.bump_distance;if(!b.dist)b.dist=20;b.tof=b.dist;if(b.direction=="down"||b.direction=="right")b.tof=-b.dist;b.slow=ss.bump_decelerator;if(!b.slow)b.slow=0;b.tb="top";b.wh="Height";if(b.direction=="left"||b.direction=="right"){b.tb="left";b.wh="Width";}b.steps=b.dist/b.frames;if(b.steps<=.5)b.steps=.51;b.orig_pos=parseInt(a.style[b.tb]);b.pos=parseInt(a.style[b.tb])+b.tof;a.style[b.tb]=b.pos+"px";a.isrun=true;var id=qm_bump_am(b);qb.pid=id;qb.pobj=a;qm_bump_ai(id);};function qm_bump_ai(id){var a=qmad.bump["_"+id];if(!a)return;var pos=parseInt(a.obj.style[a.tb]);var go=false;if(a.tof<0){if(pos+a.steps<a.orig_pos){a.obj.style[a.tb]=Math.round(pos+a.steps)+"px";go=true;}}else {if(pos-a.steps>a.orig_pos){a.obj.style[a.tb]=Math.round(pos-a.steps)+"px";go=true;}}if(go)a.timer=setTimeout("qm_bump_ai("+id+")",10);else {a.obj.style[a.tb]=a.orig_pos+"px";qmad.bump["_"+id]=null;a.obj.isrun=false;if(window.showHelp&&window.qm_over_select)qm_over_select(a.obj)}};function qm_bump_am(obj){var k;for(k in qmad.bump){if(qmad.bump[k]&&obj.obj==qmad.bump[k].obj){if(qmad.bump[k].timer){clearTimeout(qmad.bump[k].timer);qmad.bump[k].timer=null;}obj.top=qmad.bump[k].top;qmad.bump[k].obj.isrun=false;qmad.bump[k]=null;}}var i=0;while(qmad.bump["_"+i])i++;qmad.bump["_"+i]=obj;return i;};function qm_bump_is_prev_or_next(ci,compare){var nn=ci.nextSibling;while(nn){if(nn==compare)return "before";else nn=nn.nextSibling;}var nn=ci.previousSibling;while(nn){if(nn==compare)return "after";else nn=nn.previousSibling;}return false;}/* ]]> */</script>
 <!-- Add-On Code: IE Over Select Fix -->
 <script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
+<!-- Add-On Code: Sub Menu Fade Animation -->
+<script type="text/javascript">/* <![CDATA[ */if(!qmad.fade){qmad.fade=new Object();qmad.fade.iefilter=window.showHelp&&!(navigator.userAgent.indexOf("MSIE 8")+1);qmad.fade.track=new Object();qmad.fade.tracktimer=new Object();if(qmad.bvis.indexOf("qm_fade_a(b.cdiv);")==-1){qmad.bvis+="qm_fade_a(b.cdiv);";qmad.bhide+="qm_fade_a(a,1);";}};function qm_fade_a(a,hide){var z;if((a.style.visibility=="inherit"&&!hide&&!a.qmtree)||((z=window.qmv)&&(z=z.addons)&&(z=z.fade)&&!z["on"+qm_index(a)]))return;if(a.qmtreecollapse)hide=true;if(a.fftrackid)return;var ss;if(!a.settingsid){var v=a;while((v=v[qp])){if(v[qc].indexOf("qmmc")+1){a.settingsid=v.id;break;}}}ss=qmad[a.settingsid];var t;var u;if(ss&&((t=ss.fade_in_frames)||(u=ss.fade_out_frames))){if(t==null||t==undefined)t=u;if(u==null||u==undefined)u=t;a.ffinc=1/t;a.ffdec=1/u;if(!hide)a.ffcur=0;else {a.ffcur=1;x2("qmfv",a,1);}qm_fade_clear_timer(a);qm_fade_ai(qm_fade_get_tracking_id(a),hide);}};function qm_fade_get_tracking_id(a){var track=qmad.fade.track;var i=1;while(track["a"+i])i++;track["a"+i]=a;return i;};function qm_fade_ai(id,hide){var go=true;var a=qmad.fade.track["a"+id];if(!hide){a.ffcur+=a.ffinc;if(a.ffcur>1){a.ffcur=1;go=false;}}else {a.ffcur -=a.ffdec;if(a.ffcur<.001){a.ffcur=0;go=false;}}qm_fade_setfade(a,a.ffcur);if(go){qmad.fade.tracktimer["a"+id]=setTimeout("qm_fade_ai("+id+","+hide+")",10);a.fftrackid=id;}else {if(hide)x2("qmfv",a);if(qmad.fade.iefilter)a.style.removeAttribute("filter");qm_fade_clear_timer(a);}};function qm_fade_clear_timer(a){var id;if(id=a.fftrackid){clearTimeout(qmad.fade.tracktimer["a"+id]);qmad.fade.tracktimer["a"+id]=null;qmad.fade.track["a"+id]=null;a.fftrackid=null;}};function qm_fade_setfade(a,val){if(qmad.fade.iefilter)a.style.filter="alpha(opacity="+val*100+")";else a.style.opacity=val;}/* ]]> */</script>
 </head>
@@ Line 211: / Line 226: @@
 <ul id="qm0" class="qmmc">
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge">Home</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge" style="width:20px;">Home</a>
-		<ul style="width:100px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery">Project Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge#Our_Project:_The_BioBattery" style="width:90px;">Project Overview</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge#University_of_Lethbridge_Sponsors">Our Sponsors</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Team">Team</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Team" style="width:3px;">Team</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project">Project</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Project" style="width:10px;">Project</a>
-		<ul style="width:110px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#Project_Details">Project Details</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Project#The_Experiments">Experiments</a></li>
@@ Line 227: / Line 242: @@
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics">Ethics</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Ethics" style="width:40px;">Ethics and Safety</a>
-		<ul style="width:102px;">
+		<ul>
-		<li><a href="httphttps://2009.igem.org/Team:Lethbridge/Ethics#Overview">Overview</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Ethics_and_SynBio" style="width:80px;">Ethics and Synbio</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#The_Survey">The Survey</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Results">Results</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Safety">Safety</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Ethics#Analysis">Analysis</a></li>
+                </ul></li>
-		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook">Notebook</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Notebook" style="width:3px;">Notebook</a>
-		<ul style="width:132px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#Calendar">Calendar</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Notebook#May">May</a></li>
@@ Line 248: / Line 262: @@
 		</ul></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings">Meetings</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Team_Meetings" style="width:10px;">Meetings</a>
-		<ul style="width:128px;">
+		<ul>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#April">April</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#May">May</a></li>
@@ Line 258: / Line 272: @@
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#September">September</a></li>
 		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#October">October</a></li>
-		</ul></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Team_Meetings#Events">Events</a></li>
+                </ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling">Modeling</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Modeling" style="width:10px;">Modeling</a></li>
-	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts">Parts</a>
+	<li><a class="qmparent" href="https://2009.igem.org/Team:Lethbridge/Parts" style="width:10px;">Parts</a>
-		<ul style="width:93px;">
+		<ul>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_1-10">Parts 1-10</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Favourites">Favourites</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_11-20">Parts 11-20</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Individual_Parts">Individual Parts</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Parts_21-31">Parts 21-31</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Constructs.2FDevices">Constructs</a></li>
-		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediate Parts</a></li>
+		<li><a href="https://2009.igem.org/Team:Lethbridge/Parts#Intermediate_Parts">Intermediates</a></li>
 		</ul></li>
-	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria">Judging</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Collaboration" style="width:25px;">Collaboration</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/Criteria" style="width:1px;">Judging</a></li>
+	<li><a href="https://2009.igem.org/Team:Lethbridge/videos" style="width:117px;">Extra Information on SynBio</a></li>
 <li class="qmclear">&nbsp;</li></ul>
 <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
+<script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 </body>
@@ Line 279: / Line 297: @@
 [[Image:Igembanner.jpg|965px|]]
+==Project Overview==
-<br />
+[[Image:Biobattery.png|right|275px]]
-{|align="justify"
-|-
-|
-''
+The issues surrounding energy production are becoming more prominent with increasing environmental concerns and the rising cost of energy.  Microbial fuel cells (MFCs) use biological systems to produce an electrical current.  Cyanobacteria are organisms which have been studied in MFCs and have been found to create a current, although not highly efficient (Tsujimura et al., 2001).
-== Our Project: The BioBattery ==
+We wish to increase the efficiency of the cyanobacteria MFC by introducing microcompartments to create a BioBattery.  The microcompartments are created by the production of the protein lumazine synthase forms icosahedral capsids.   As a proof of principle we will create this system within Escherichia coli and target two different fluorescent proteins within the microcompartment to observe fluorescence resonance energy transfer.  Furthermore, we will be exploring a novel method for the mass production of uniform nanoparticles, which is more efficient and cost effective than current methods.
+'''References'''
-With increasing concerns surrounding the environment and rising energy costs, many are devoting more time to address the issues surrounding energy production. In the 2009 Canadian Federal Budget, one billion dollars was designated to develop sustainable energy projects. In keeping with this, the University of Lethbridge iGEM Team is working towards creating an efficient sustainable energy source, the BioBattery.
+Tsujimura, S., Wadano, A., Kano, K., and Ikeda, T., (2001). Photosynthetic
+bioelectrochemical cell utilizing cyanobacteria and water-generating
+oxidase. Enzyme and Microbial Technology. 29, 225-231
-The BioBattery is based on using photosynthetic pathways to create energy.  The self-sustaining battery will fix carbon dioxide from the atmosphere, provided that it is supplied with water and sunlight. However, simply using these photosynthetic pathways will not create a BioBattery efficient enough to be economically and environmentally applicable. Therefore, the creation of synthetic microcompartments is necessary to increase the energy output of the BioBattery. The microcompartments can contain the photosynthetic factors needed to maximize BioBattery efficiency.
+==University of Lethbridge Sponsors==
-''
-== University of Lethbridge Sponsors ==
+<html>
+<body>
 <html>
 <body>
+<table width="965" border="0.5">
+ <tr>
+<td>
 <a href="http://www.uleth.ca" target="_blank">
-<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_fiat_143.jpg"></a>
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_fiat_143.jpg"></a></td>
+<td>
+<a href="http://www.albertaingenuity.ca/international/genetically/engineered/machines/igem" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/AlbertaIngenuitylogo.jpg"></a></td>
+<td><a href="http://www.rainin.com/canada/default_can.asp" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/RAININ_Logo_Japan_big.jpg"></a>
+</td>
+</tr>
+<tr>
+<td><a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
+<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_mrgene.jpg"></a></td>
+<td>
+<a href="http://www.viterra.ca/portal/wps/portal/viterra" target="_blank">
+<img src="http://s904.photobucket.com/albums/ac246/MegLand/Viterra.jpg"></a>
+</td>
+<td>
+<a href="http://www.vwrcanlab.com/" target="_blank">
+<img src="http://s904.photobucket.com/albums/ac246/MegLand/VWR.jpg"></a></td>
+</tr>
+</table>
-<a href="http://mrgene.com/desktopdefault.aspx/tabid-2/" target="_blank">
+</body>
-<img src="http://i770.photobucket.com/albums/xx343/ulethigem/logo_mrgene.jpg"></a>
+</html>
-<a href="http://www.albertaingenuity.ca/international/genetically/engineered/machines/igem" target="_blank">
-<img src="http://i770.photobucket.com/albums/xx343/ulethigem/AlbertaIngenuity.gif"></a>
-</body>
+<html>
+<head>
+<html>
 <head>
 <!--%%%%%%%%%%%% QuickMenu Styles [Keep in head for full validation!] %%%%%%%%%%%-->
@@ Line 341: / Line 384: @@
 		padding:5px 60px 3px 0px;
 		margin:0px 5px 0px 0px;
-		color:#FFAA00;
+		color:#FFD600;
 		font-family:Arial;
 		font-size:12px;
@@ Line 348: / Line 391: @@
 		border-width:0px 0px 8px 0px;
 		border-style:solid;
-		border-color:#FFAA00;
+		border-color:#FFD600;
 	}
@@ Line 355: / Line 398: @@
 	#qm0 a:hover
 	{
-		color:#006363;
+		color:#3C13AF;
-		border-color:#FF0000;
+		border-color:#FFAD00;
 	}
@@ Line 363: / Line 406: @@
 	#qm0 li:hover>a
 	{
-		color:#006363;
+		color:#3C13AF;
-		border-color:#FF0000;
+		border-color:#FFAD00;
 	}
@@ Line 379: / Line 422: @@
 	body #qm0 .qmactive, body #qm0 .qmactive:hover
 	{
-		color:#006363;
+		color:#3C13AF;
 		text-decoration:none;
-		border-color:#FF0000;
+		border-color:#FFAD00;
 	}
@@ Line 392: / Line 435: @@
 		border-width:1px;
 		border-style:solid;
-		border-color:#FF0000;
+		border-color:#3C13AF;
 	}
@@ Line 401: / Line 444: @@
 		padding:3px 5px 3px 5px;
 		background-color:transparent;
-		color:#000000;
+		color:#230672;
 		font-size:11px;
 		font-weight:normal;
 		border-width:0px;
 		border-style:none;
-		border-color:#000000;
+		border-color:#230672;
 	}
@@ Line 413: / Line 456: @@
 	#qm0 div a:hover
 	{
-		background-color:#666666;
+		background-color:#476DD5;
 		color:#FFFFFF;
 		text-decoration:none;
@@ Line 422: / Line 465: @@
 	#qm0 ul li:hover>a
 	{
-		background-color:#666666;
+		background-color:#476DD5;
 		color:#FFFFFF;
 		text-decoration:none;
@@ Line 438: / Line 481: @@
 	body #qm0 div .qmactive, body #qm0 div .qmactive:hover
 	{
-		background-color:#666666;
+		background-color:#476DD5;
 		background-image:url(qmimages/arrow_1.gif);
 		color:#FFFFFF;
@@ Line 476: / Line 519: @@
 		background-image:url(qmimages/arrow_1.gif);
 	}
 </style>
 <!-- Add-On Core Code (Remove when not using any add-on's) -->
@@ Line 492: / Line 533: @@
 		// Match Widths Add On
 		a.mwidths_active = true;
+		// IE Over Select Fix Add On
+		a.overselects_active = true;
 	</script>
@@ Line 501: / Line 545: @@
 <script type="text/javascript">/* <![CDATA[ */qmad.mwidths=new Object();if(qmad.bvis.indexOf("qm_mwidths_a(b.cdiv,o);")==-1)qmad.bvis+="qm_mwidths_a(b.cdiv,o);";;function qm_mwidths_a(sub,item){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.match_widths)&&!z["on"+qm_index(sub)])return;var ss;if(!item.settingsid){var v=item;while((v=v.parentNode)){if(v.className.indexOf("qmmc")+1){item.settingsid=v.id;break;}}}ss=qmad[item.settingsid];if(!ss)return;if(!ss.mwidths_active)return;if(qm_a(item.parentNode)){var t=0;t+=qm_getcomputedstyle(sub,"padding-left","paddingLeft");t+=qm_getcomputedstyle(sub,"padding-right","paddingRight");t+=qm_getcomputedstyle(sub,"border-left-width","borderLeftWidth");t+=qm_getcomputedstyle(sub,"border-right-width","borderRightWidth");var adj=0;adj=item.getAttribute("matchwidthadjust");if(adj)adj=parseInt(adj);if(!adj||isNaN(adj))adj=0;sub.style.width=(item.offsetWidth-t+adj)+"px";var a=sub.childNodes;for(var i=0;i<a.length;i++){if(a[i].tagName=="A")a[i].style.whiteSpace="normal";}}};function qm_getcomputedstyle(obj,sname,jname){var v;if(document.defaultView&&document.defaultView.getComputedStyle)v=document.defaultView.getComputedStyle(obj,null).getPropertyValue(sname);else  if(obj.currentStyle)v=obj.currentStyle[jname];if(v&&!isNaN(v=parseInt(v)))return v;else return 0;}/* ]]> */</script>
-</head>
+<!-- Add-On Code: IE Over Select Fix -->
+<script type="text/javascript">/* <![CDATA[ */if(window.showHelp&&!window.XMLHttpRequest){if(qmad.bvis.indexOf("qm_over_select(b.cdiv);")==-1){qmad.bvis+="qm_over_select(b.cdiv);";qmad.bhide+="qm_over_select(a,1);";}};function qm_over_select(a,hide){var z;if((z=window.qmv)&&(z=z.addons)&&(z=z.over_select)&&!z["on"+qm_index(a)])return;if(!a.settingsid){var v=a;while(!qm_a(v))v=v[qp];a.settingsid=v.id;}var ss=qmad[a.settingsid];if(!ss)return;if(!ss.overselects_active)return;if(!hide&&!a.hasselectfix){var f=document.createElement("IFRAME");f.style.position="absolute";f.style.filter="alpha(opacity=0)";f.src="javascript:false;";f=a.parentNode.appendChild(f);f.frameborder=0;a.hasselectfix=f;}var b=a.hasselectfix;if(b){if(hide)b.style.display="none";else {var oxy=0;if(a.hasshadow&&a.hasshadow.style.visibility=="inherit")oxy=parseInt(ss.shadow_offset);if(!oxy)oxy=0;b.style.width=a.offsetWidth+oxy;b.style.height=a.offsetHeight+oxy;b.style.top=a.style.top;b.style.left=a.style.left;b.style.margin=a.currentStyle.margin;b.style.display="block";}}}/* ]]> */</script>
+</head>
 <body>
 <ul id="qm0" class="qmmc">
@@ Line 514: / Line 558: @@
 <!-- Create Menu Settings: (Menu ID, Is Vertical, Show Timer, Hide Timer, On Click (options: 'all' * 'all-always-open' * 'main' * 'lev2'), Right to Left, Horizontal Subs, Flush Left, Flush Top) -->
 <script type="text/javascript">qm_create(0,false,0,500,false,false,false,false,false);</script>
 </body>
 </html>
+==University of Lethbridge Supporters==
+The University of Lethbridge iGEM Team would like to thank the many individuals who have given us their time, money or support.  Including, but not limited to:
+The Wieden Lab
+The Kothe Lab
+The Selinger Lab
+The Biochemistry Teaching Lab and coordinators
+The University of Lethbridge Student's Union
+David Franz
+Nathan Puhl
+John Thibault
+Sebastian Machula
+and
+Tony Russell