Team:Cornell/Parts

From 2009.igem.org

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{|align="justify"
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=== Device ===
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{| style="border:1px solid #cef2e0; background:#f5fffa; color:#008811; vertical-align:top;" cellpadding="3" cellspacing="1" width="900px" align="center"
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!align="center"|[[Team:Cornell|The Project]]
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!align="center"|[[Team:Cornell/Project/Background|Background]]
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!align="center"|[[Team:Cornell/Project/Chassis|Chassis]]
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!align="center"|[[Team:Cornell/Project/Design|Design]]
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!align="center"|[[Team:Cornell/Project/Standardization|Standardization]]
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!align="center"|[[Team:Cornell/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Cornell/Notebook|Notebook]]
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!align="center"|[[Team:Cornell/Team|The Team]]
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=Parts=
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===Device===
''Cadmium Sensor (BBa_K285104)''
''Cadmium Sensor (BBa_K285104)''
expresses GFP as a function of Cadmium Ion concentration. The first part of the GFP Cadmium Sensor is the CadA regulatory element which increases expression in response increasing Cadmium concentration. This is followed by spoVG a ribosome binding site for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis.  
expresses GFP as a function of Cadmium Ion concentration. The first part of the GFP Cadmium Sensor is the CadA regulatory element which increases expression in response increasing Cadmium concentration. This is followed by spoVG a ribosome binding site for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis.  
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[[Image:Module 1 Active.GIF|600px|center]]
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[[Image:Module 1 Active.GIF|600px|center]]
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=== Individual Parts===
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''CadA Regulatory Region (BBa_K285101,[http://partsregistry.org/Part:BBa_K285101:Design])'' is the regulatory sequence upstream of the cadA gene (previously known as yvgW). This sequence is part of the czrA regulon and is regulated by the CzrA repressor which is derepressed in the presence of Cd2+.[1][2]
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''copZA Regulatory Region (BBa_K285102,[http://partsregistry.org/Part:BBa_K285102:Design])'' is the regulatory sequence upstream of the copZ and copA genes. Activation of the copZ gene proceeds by copper mediated induction by the CueR protein which binds to this region.[2]
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''mntH Regulatory Region (BBa_K285103,[http://partsregistry.org/Part:BBa_K285103:Design])''is upstream of the mntH gene is repressed by the MntR protein which is derepressed upon induction by Cd2+.[1][3]
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== Individual Parts==
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=== References===
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''CadA Regulatory Region (BBa_K285101)'' is
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[1]Moore CM, Gaballa A, Hui M, Ye RW, Helmann JD. Genetic and physiological responses of Bacillus subtilis to metal ion stress. Mol Microbiol. 2005 Jul;57(1):27-40.
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''copZA Regulatory Region (BBa_K285102)'' is bound by CueR protein in the presence of Copper activating the copZ gene. The copZA regulatory region is previously known as yvgY.
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[2]Ahmed Gaballa & John D. Helmann Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems. Biometals. 2003 Dec;16(4):497-505.  
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''mntH Regulatory Region (BBa_K285103)''is upstream of the mntH gene is repressed by the MntR protein which is derepressed upon induction by Cd2+.
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[3]Que Q, Helmann JD.Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins.Mol Microbiol. 2000 Mar;35(6):1454-68.

Latest revision as of 03:58, 22 October 2009

CUGEM Banner.png
The Project Background Chassis Design Standardization Parts Submitted to the Registry Notebook The Team

Contents

Parts

Device

Cadmium Sensor (BBa_K285104) expresses GFP as a function of Cadmium Ion concentration. The first part of the GFP Cadmium Sensor is the CadA regulatory element which increases expression in response increasing Cadmium concentration. This is followed by spoVG a ribosome binding site for Bacillus Subtilis. The GFP coding sequence follows. Note that the GFP Cadmium Sensor will only function with the czrA regulon found in Bacillus Subtillis.

Module 1 Active.GIF

Individual Parts

CadA Regulatory Region (BBa_K285101,[http://partsregistry.org/Part:BBa_K285101:Design]) is the regulatory sequence upstream of the cadA gene (previously known as yvgW). This sequence is part of the czrA regulon and is regulated by the CzrA repressor which is derepressed in the presence of Cd2+.[1][2]

copZA Regulatory Region (BBa_K285102,[http://partsregistry.org/Part:BBa_K285102:Design]) is the regulatory sequence upstream of the copZ and copA genes. Activation of the copZ gene proceeds by copper mediated induction by the CueR protein which binds to this region.[2]

mntH Regulatory Region (BBa_K285103,[http://partsregistry.org/Part:BBa_K285103:Design])is upstream of the mntH gene is repressed by the MntR protein which is derepressed upon induction by Cd2+.[1][3]

References

[1]Moore CM, Gaballa A, Hui M, Ye RW, Helmann JD. Genetic and physiological responses of Bacillus subtilis to metal ion stress. Mol Microbiol. 2005 Jul;57(1):27-40.

[2]Ahmed Gaballa & John D. Helmann Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems. Biometals. 2003 Dec;16(4):497-505.

[3]Que Q, Helmann JD.Manganese homeostasis in Bacillus subtilis is regulated by MntR, a bifunctional regulator related to the diphtheria toxin repressor family of proteins.Mol Microbiol. 2000 Mar;35(6):1454-68.

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