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Team:Heidelberg/Parts
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{| | {| | ||
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| - | |width="650px" style="padding: 0 20px | + | |width="650px" style="padding: 0 15px 15px 20px; background-color:#ede8e2"| |
__NOTOC__ | __NOTOC__ | ||
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= Parts = | = Parts = | ||
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== Measurement Kit == | == Measurement Kit == | ||
| + | [[Team:Heidelberg/Project_Measurement|''Main Article: Measurement'']] | ||
{| class="wikitable centered" style="margin: 1em auto 1em auto" | {| class="wikitable centered" style="margin: 1em auto 1em auto" | ||
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== Core promoters == | == Core promoters == | ||
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| + | [[Team:Heidelberg/Project_Measurement#Different_core_promoters_result_in_different_expression_strength|''Main Article: Core promoters'']] | ||
The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]]. | The following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by [[Project_Measurement#Different_core_promoters_result_in_different_expression_strength|core promoter swapping]]. | ||
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| - | == Promoters created by [[Team:Heidelberg/Project_Synthetic_promoters| | + | == Promoters created by RA-PCR == |
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| + | [[Team:Heidelberg/Project_Synthetic_promoters|''Main article: Synthetic promoters'']] | ||
All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]. | All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100]. | ||
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== HEARTBEAT Predicted promoters == | == HEARTBEAT Predicted promoters == | ||
| - | + | [[Team:Heidelberg/HEARTBEAT_database| ''Main article: HEARTBEAT Database'']] | |
| - | ''' | + | {| class="wikitable centered" style="margin: 1em auto 1em auto" |
| + | |- style="background-color:#99cccc;" | ||
| + | ! height=20px, width=250px | Name || width=150px | Part type || width=150 | Registry link | ||
| + | |- style="background-color:#9BDDFF;" | ||
| + | | style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB8 || Regulatory || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203125 Part:BBa_K203125 ] | ||
| + | |- style="background-color:#9BDDFF;" | ||
| + | | style="font-weight:bold;" |SREBP regulated promoter, predicted, tested HB9 || Regulatory || [http://partsregistry.org/Part:BBa_K203126 Part:BBa_K203126] | ||
| + | |- | ||
| + | |} | ||
== Natural promoters == | == Natural promoters == | ||
| + | |||
| + | [[Team:Heidelberg/Project_Natural_promoters|''Main article: Natural promoters'']] | ||
{| class="wikitable centered" style="margin: 1em auto 1em auto" | {| class="wikitable centered" style="margin: 1em auto 1em auto" | ||
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== Targeting Signals == | == Targeting Signals == | ||
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| + | [[Team:Heidelberg/Project_SaO|''Main article: Outlook and Summary'']] | ||
Protein domains for targeting to sub-cellular compartments. | Protein domains for targeting to sub-cellular compartments. | ||
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|- style="background-color:#9BDDFF;" | |- style="background-color:#9BDDFF;" | ||
| style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116] | | style="font-weight:bold;" | mCherry-myrpalm-polyA || Composite || [http://partsregistry.org/Part:BBa_K203116 Part:BBa_K203116] | ||
| + | |- | ||
| + | |- style="background-color:#9BDDFF;" | ||
| + | | style="font-weight:bold;" | GFP-polyA || Composite || [http://partsregistry.org/Part:BBa_K203117 Part:BBa_K203117] | ||
|- | |- | ||
|} | |} | ||
| - | |width="250px" style="background-color:#d8d5d0"| | + | |width="250px" style="padding: 0 20px 15px 15px; background-color:#d8d5d0"| |
|} | |} | ||
Latest revision as of 09:06, 4 July 2011
PartsMeasurement Kit
Core promotersThe following Parts contain the JeT proximal promoter, and a core promoter as described. Core and proximal promoter are separated by a HindIII site. Parts can be applied for testing synthetic proximal promoters. The strength of promoters derived from these parts can be modified by core promoter swapping.
Promoters created by RA-PCRMain article: Synthetic promoters All contain the core promoter of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203112 Part:BBa_K203112] and were screened / measured in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K203100 Part:BBa_K203100].
HEARTBEAT Predicted promotersMain article: HEARTBEAT Database
Natural promotersMain article: Natural promoters
Targeting SignalsMain article: Outlook and Summary Protein domains for targeting to sub-cellular compartments.
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