Team:UNIPV-Pavia/Protocols/Precipitation

From 2009.igem.org

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(→DNA precipitation with sodium acetate)
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*We use it only if the miniprep did not give us a high yield of DNA
 
*Add 1/10 digestion volume of sodium acetate 3 M
*Add 1/10 digestion volume of sodium acetate 3 M
*Add 2.5 digestion volume of absolute ethanol
*Add 2.5 digestion volume of absolute ethanol

Revision as of 20:52, 9 July 2009

EthanolPVanimation.gif



Protocols

DNA precipitation with sodium acetate

(estimated time: 1 hour)

  • Add 1/10 digestion volume of sodium acetate 3 M
  • Add 2.5 digestion volume of absolute ethanol
  • Freeze at -80°C for 30 min
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Decant supernatant
  • Add 50 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4°C for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5 µl ddH2O




  • Sodium acetate
  • Absolute ethanol
  • Ethanol 70%
  • ddH2O
  • Previously cut plasmid




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