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Team:UNIPV-Pavia/Protocols/Precipitation
From 2009.igem.org
(Difference between revisions)
(→DNA precipitation with sodium acetate) |
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<table width='80%'><tr><td> | <table width='80%'><tr><td> | ||
| - | *Add 1/10 | + | *Add 1/10 DNA solution volume of sodium acetate 3 M |
| - | *Add 2.5 | + | *Add 2.5 DNA solution volume of absolute ethanol |
*Freeze at -80°C for 30 min | *Freeze at -80°C for 30 min | ||
*Centrifuge at 13000 rpm, 4°C for 20 min | *Centrifuge at 13000 rpm, 4°C for 20 min | ||
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*Remove all supernatant with a pipette | *Remove all supernatant with a pipette | ||
*Air dry pellet until ethanol is totally removed | *Air dry pellet until ethanol is totally removed | ||
| - | *Elute with 5 µl ddH2O | + | *Elute with 5-10 µl of ddH2O |
<br><br> | <br><br> | ||
<br></td><td align='center' valign='bottom' width='300px'> | <br></td><td align='center' valign='bottom' width='300px'> | ||
Revision as of 20:55, 9 July 2009
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DNA precipitation with sodium acetate
(estimated time: 1 hour)
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