Team:Groningen/Brainstorm/Rainbow Bacteria

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Revision as of 19:43, 27 April 2009

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Tianjin University 2008 (China) – Made use of the CRE/LoxP combination (together with other strategies) to combine DNA and make “Foolproof Plasmid Self-Assembly Systems”.

MIT 2006 (USA) – Mention of mutant LoxP sites to prevent inversion of sequences (Lox66 and Lox71)

Imperial College 2006 (London) – Biological oscillator by using the CRE/Lox system as a switch by incorporating DNA to prevent downstream transcription. In the end used Quorum Sensing to create a predator – pray system.

Paris 2007 (France) – (Synthetic Multicellular Bacterium) Use of CRE/Lox system to influence differentiation in germ line, because it was readily available, largely used and well described. Low concentrations of DAP triggered the transcription of Cre-recombinase (under the influence of DAP-sensitive promoter), which caused the removal of a gene required for bacterial reproduction by site-specific recombination (SSR), and resulted in the activation of a gene for the production of DAP by them. The SSR sites might become a burden.

Berkeley 2007 (England) – Registration of recombinase parts (Cre), Bacteriophage P1-derived Cre-Lox.

USTC (China) – made use of Cre to cut out “sender” genes upon receiving a signal from a sender cell and thereby creating a receiver cell.

Tsinghua – use of the Cre/Lox system in their second project, but is unclear why they used it.

Utah State University – use of GFP as a reporter gene to indicate the maximum production level of PHB.

University of Sheffield – use of GFP as a reporter gene upon detection of a pathogen by a receiver protein.

UNIPV-Pavia – use of GFP and RFP as reporter genes in electronically based circuit (on/off).

TUDelft – use of different method to produce colors, but results in this area were not very promising (also due to ordered parts from iGEM).

Tokyo Tech – use of GFP as a reporter gene in their “coli touch” display experiment.

Paris – use of (E)CFP, YFP, GFP and mRFP in the construction of a oscillating time clock project.

Newcastle University – use of a XFP as a reporter gene to indicate the amount of increase in POP’s.

Fluorescent protein sequences

Workable are the wild-type GFP (and a few mutants), RFP (and cherry version)
Not-workable (no info) are YFP and CFP (yellow and cyan), OFP (orange), SBFP2 (blue) and a few mutant proteins.

Recombinase

Workable are Cre DNA recombinase, and Hin invertase
No info (not-workable) are mutant (altered stop/start codon) Cre versions, and a few other enzymes like integrase.

Recombination sites

Membrane proteins

Only a few mentioned in the lists of receptors, transporters, channels, pumps and “other proteins”, maybe 40 in total, but I am not familiar with these names (TLR, Omp).

All of the workable protein sequences are listed as “1 star” in the availability list!!

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+!align="center"|[[Team:Groningen|Home]]
+!align="center"|[[Team:Groningen/Team|The Team]]
+!align="center"|[[Team:Groningen/Brainstorm|Brainstorm]]
+!align="center"|[[Team:Groningen/Vision|Our Vision]]
+!align="center"|[[Team:Groningen/Parts|Parts]]
+!align="center"|[[Team:Groningen/Modelling|Modelling]]
+!align="center"|[[Team:Groningen/Notebook|Notebook]]
+|}
 ==Use or mention of the Cre/Lox System==