Team:Groningen/Brainstorm/Rainbow Bacteria
From 2009.igem.org
(Difference between revisions)
(Addition of nevigation bar) |
(Added links for better navigation) |
||
Line 12: | Line 12: | ||
==Use or mention of the Cre/Lox System== | ==Use or mention of the Cre/Lox System== | ||
- | :*Tianjin University 2008 (China) – Made use of the CRE/LoxP combination (together with other strategies) to combine DNA and make “Foolproof Plasmid Self-Assembly Systems”. | + | :*[https://2008.igem.org/Team:Tianjin Tianjin University] 2008 (China) – Made use of the CRE/LoxP combination (together with other strategies) to combine DNA and make “Foolproof Plasmid Self-Assembly Systems”. |
:*MIT 2006 (USA) – Mention of mutant LoxP sites to prevent inversion of sequences (Lox66 and Lox71) | :*MIT 2006 (USA) – Mention of mutant LoxP sites to prevent inversion of sequences (Lox66 and Lox71) | ||
Line 22: | Line 22: | ||
:*Berkeley 2007 (England) – Registration of recombinase parts (Cre), Bacteriophage P1-derived Cre-Lox. | :*Berkeley 2007 (England) – Registration of recombinase parts (Cre), Bacteriophage P1-derived Cre-Lox. | ||
- | :*USTC (China) – made use of Cre to cut out “sender” genes upon receiving a signal from a sender cell and thereby creating a receiver cell. | + | :*[https://2008.igem.org/Team:USTC USTC] 2008 (China) – made use of Cre to cut out “sender” genes upon receiving a signal from a sender cell and thereby creating a receiver cell. |
- | :*Tsinghua – use of the Cre/Lox system in their second project, but is unclear why they used it. | + | :*[https://2008.igem.org/Team:Tsinghua Tsinghua] 2008 – use of the Cre/Lox system in their second project, but is unclear why they used it. |
==Use or mention of XFP’s== | ==Use or mention of XFP’s== | ||
- | :*Utah State University – use of GFP as a reporter gene to indicate the maximum production level of PHB. | + | :*[https://2008.igem.org/Team:Utah_State Utah State University] – use of GFP as a reporter gene to indicate the maximum production level of PHB. |
- | :*University of Sheffield – use of GFP as a reporter gene upon detection of a pathogen by a receiver protein. | + | :*[https://2008.igem.org/Team:University_of_Sheffield University of Sheffield] – use of GFP as a reporter gene upon detection of a pathogen by a receiver protein. |
- | :*UNIPV-Pavia – use of GFP and RFP as reporter genes in electronically based circuit (on/off). | + | :*[https://2008.igem.org/Team:UNIPV-Pavia UNIPV-Pavia] – use of GFP and RFP as reporter genes in electronically based circuit (on/off). |
- | :*UCSF – use of GFP as a reporter gene in DNA silencing by Chromatin. | + | :*[https://2008.igem.org/Team:UCSF UCSF] – use of GFP as a reporter gene in DNA silencing by Chromatin. |
- | :*TUDelft – use of different method to produce colors, but results in this area were not very promising (also due to ordered parts from iGEM). | + | :*[https://2008.igem.org/Team:TUDelft TUDelft] – use of different method to produce colors, but results in this area were not very promising (also due to ordered parts from iGEM). |
- | :*Tokyo Tech – use of GFP as a reporter gene in their “coli touch” display experiment. | + | :*[https://2008.igem.org/Team:Tokyo_Tech Tokyo Tech] – use of GFP as a reporter gene in their “coli touch” display experiment. |
- | :*Paris – use of (E)CFP, YFP, GFP and mRFP in the construction of a oscillating time clock project. | + | :*[https://2008.igem.org/Team:Paris Paris] – use of (E)CFP, YFP, GFP and mRFP in the construction of a oscillating time clock project. |
- | :*Newcastle University – use of a XFP as a reporter gene to indicate the amount of increase in POP’s. | + | :*[https://2008.igem.org/Team:Newcastle_University Newcastle University] – use of a XFP as a reporter gene to indicate the amount of increase in POP’s. |
- | :*Missouri University – use of RFP with special introduced promoter. | + | :*[https://2008.igem.org/Team:Missouri_Miners Missouri University] – use of RFP with special introduced promoter. |
- | :*Minnesota – use of GFP and RFP as reporter genes. | + | :*[https://2008.igem.org/Team:Minnesota Minnesota] – use of GFP and RFP as reporter genes. |
- | :*Bologna – use of GFP and RFP as reporter genes. | + | :*[https://2008.igem.org/Team:Bologna Bologna] – use of GFP and RFP as reporter genes. |
- | ==Parts in the Registry:== | + | ==Parts in the [http://partsregistry.org/Main_Page Registry of Standard Parts]:== |
'''Fluorescent protein sequences''' | '''Fluorescent protein sequences''' |
Revision as of 20:09, 27 April 2009
Home | The Team | Brainstorm | Our Vision | Parts | Modelling | Notebook |
---|
Use or mention of the Cre/Lox System
- Tianjin University 2008 (China) – Made use of the CRE/LoxP combination (together with other strategies) to combine DNA and make “Foolproof Plasmid Self-Assembly Systems”.
- MIT 2006 (USA) – Mention of mutant LoxP sites to prevent inversion of sequences (Lox66 and Lox71)
- Imperial College 2006 (London) – Biological oscillator by using the CRE/Lox system as a switch by incorporating DNA to prevent downstream transcription. In the end used Quorum Sensing to create a predator – pray system.
- Paris 2007 (France) – (Synthetic Multicellular Bacterium) Use of CRE/Lox system to influence differentiation in germ line, because it was readily available, largely used and well described. Low concentrations of DAP triggered the transcription of Cre-recombinase (under the influence of DAP-sensitive promoter), which caused the removal of a gene required for bacterial reproduction by site-specific recombination (SSR), and resulted in the activation of a gene for the production of DAP by them. The SSR sites might become a burden.
- Berkeley 2007 (England) – Registration of recombinase parts (Cre), Bacteriophage P1-derived Cre-Lox.
- USTC 2008 (China) – made use of Cre to cut out “sender” genes upon receiving a signal from a sender cell and thereby creating a receiver cell.
- Tsinghua 2008 – use of the Cre/Lox system in their second project, but is unclear why they used it.
Use or mention of XFP’s
- Utah State University – use of GFP as a reporter gene to indicate the maximum production level of PHB.
- University of Sheffield – use of GFP as a reporter gene upon detection of a pathogen by a receiver protein.
- UNIPV-Pavia – use of GFP and RFP as reporter genes in electronically based circuit (on/off).
- UCSF – use of GFP as a reporter gene in DNA silencing by Chromatin.
- TUDelft – use of different method to produce colors, but results in this area were not very promising (also due to ordered parts from iGEM).
- Tokyo Tech – use of GFP as a reporter gene in their “coli touch” display experiment.
- Paris – use of (E)CFP, YFP, GFP and mRFP in the construction of a oscillating time clock project.
- Newcastle University – use of a XFP as a reporter gene to indicate the amount of increase in POP’s.
- Missouri University – use of RFP with special introduced promoter.
- Minnesota – use of GFP and RFP as reporter genes.
- Bologna – use of GFP and RFP as reporter genes.
Parts in the [http://partsregistry.org/Main_Page Registry of Standard Parts]:
Fluorescent protein sequences
- Workable are the wild-type GFP (and a few mutants), RFP (and cherry version)
- Not-workable (no info) are YFP and CFP (yellow and cyan), OFP (orange), SBFP2 (blue) and a few mutant proteins.
Recombinase
- Workable are Cre DNA recombinase, and Hin invertase
- No info (not-workable) are mutant (altered stop/start codon) Cre versions, and a few other enzymes like integrase.
Recombination sites
- Workable is the Lox site for recombination
- No info (not-workable) are lox66 and lox71
Membrane proteins
- Only a few mentioned in the lists of receptors, transporters, channels, pumps and “other proteins”, maybe 40 in total, but I am not familiar with these names (TLR, Omp).
All of the workable protein sequences are listed as “1 star” in the availability list!!