"
Team:Wash U/Calendar-Home/1 July 2009
From 2009.igem.org
(Difference between revisions)
| (9 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{WashUback}} | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | == Wednesday July 1, 2009 == | ||
| + | |||
-Miniprep #2 to obtain more biobrick DNA | -Miniprep #2 to obtain more biobrick DNA | ||
-New gel with digested vs. undigested plasmids | -New gel with digested vs. undigested plasmids | ||
| - | -Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run | + | -Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow |
| - | + | ||
| + | -Started new overnight cultures of biobrick parts and plasmids | ||
| + | -Team meeting #4 | ||
Miniprep #2: | Miniprep #2: | ||
| + | <table> | ||
| + | <tr> | ||
| + | <td> | ||
1) BBa_I15010 pSB2K3 | 1) BBa_I15010 pSB2K3 | ||
| Line 33: | Line 44: | ||
12) plasmid pRKCBC3 | 12) plasmid pRKCBC3 | ||
| + | |||
| + | <td> | ||
| + | |||
| + | 35 ng/ul | ||
| + | |||
| + | 45 ng/ul | ||
| + | |||
| + | used | ||
| + | |||
| + | 62.5 ng/ul | ||
| + | |||
| + | 42 ng/ul | ||
| + | |||
| + | 28.5 ng/ul | ||
| + | |||
| + | 133.0 ng/ul | ||
| + | |||
| + | 62.5 ng/ul | ||
| + | |||
| + | 54.0 ng/ul | ||
| + | |||
| + | 64.5 ng/ul | ||
| + | |||
| + | 31.5 ng/ul | ||
| + | |||
| + | 45.5 ng/ul | ||
| + | |||
| + | </table> | ||
| + | |||
| + | <pre> | ||
| + | Note: Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma) | ||
| + | *Measured again with EtOH wipe after each measurement 7-15 | ||
| + | </pre> | ||
| + | |||
| + | |||
| + | <div style="text-align: right;"> | ||
| + | <div style="float: left;"> | ||
| + | [[Team:Wash_U/Calendar-Home/30 June 2009|Previous entry]]</div> | ||
| + | [[Team:Wash_U/Calendar-Home/2 July 2009|Next entry]]</div> | ||
| + | <div style="clear: left;"></div> | ||
| + | |||
| + | <center>[[Team:Wash U/Notebook|Back to Calendar]]</center> | ||
| + | |||
| + | |||
| + | |||
| + | {{WashUbottom}} | ||
Latest revision as of 21:12, 15 July 2009
