Team:Wash U/Calendar-Home/1 July 2009

From 2009.igem.org

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-Started new overnight cultures of biobrick parts and plasmids
-Started new overnight cultures of biobrick parts and plasmids
 +
-Team meeting #4
Miniprep #2:
Miniprep #2:
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<td>
<td>
-
158 ng/ul
+
35 ng/ul
-
210 ng/ul
+
45 ng/ul
 +
 
 +
used
62.5 ng/ul
62.5 ng/ul
-
325 ng/ul
+
42 ng/ul
-
280 ng/ul
+
28.5 ng/ul
-
145 ng/ul
+
133.0 ng/ul
-
665 ng/ul
+
62.5 ng/ul
-
 
+
-
308 ng/ul
+
-
275 ng/ul
+
54.0 ng/ul
-
338 ng/ul
+
64.5 ng/ul
-
168 ng/ul
+
31.5 ng/ul
-
272 ng/ul
+
45.5 ng/ul
</table>
</table>
<pre>
<pre>
-
*Note:  Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
+
Note:  Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
 +
*Measured again with EtOH wipe after each measurement 7-15
</pre>
</pre>

Latest revision as of 21:12, 15 July 2009



Wednesday July 1, 2009

-Miniprep #2 to obtain more biobrick DNA

-New gel with digested vs. undigested plasmids

-Started new restriction digests on plasmids (single cut with enzyme #1, single cut with enzyme #2, double cut); will run these on gel tomorrow

-Started new overnight cultures of biobrick parts and plasmids

-Team meeting #4

Miniprep #2:

1) BBa_I15010 pSB2K3

2) BBa_E0240 pSB1A2

3) BBa_R0082 pSB1A2

4) plasmid pSB1T3

5) plasmid pSB1K3

6) BBa_B0034 pSB1A2

7) plasmid pSB1AT3

8) plasmid pSB1A3

9) plasmid pucHS27

10) plasmid pSB1AC3

11) BBa_B0015 pSB1AK3

12) plasmid pRKCBC3

35 ng/ul

45 ng/ul

used

62.5 ng/ul

42 ng/ul

28.5 ng/ul

133.0 ng/ul

62.5 ng/ul

54.0 ng/ul

64.5 ng/ul

31.5 ng/ul

45.5 ng/ul

Note:  Nanospec: Used 3ul on Lid 10, 320nm background ON, blank after each measurement with TE elution buffer (Sigma)
*Measured again with EtOH wipe after each measurement 7-15


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