Virginia Commonwealth/16 July 2009

From 2009.igem.org

(Difference between revisions)
(→Results)
Line 1: Line 1:
 +
<div id="header">{{Template:Team:Virginia_Commonwealth/Templates/Header}}</div>
 +
<table>
 +
<html>
 +
<style type="text/css">
 +
table.calendar          { margin: 0; padding: 2px; }
 +
table.calendar td      { margin: 0; padding: 1px; vertical-align: top; }
 +
table.month .heading td { padding:0px; background-color: black; color: gold; text-align:center; font-size:100%; font-weight:bold; }
 +
table.month .dow td    { color:#000000; text-align:center; font-size:100%; }
 +
table.month td.today    { background-color:#cd0000; }
 +
table.month td {
 +
    border: none;
 +
    margin: 0;
 +
    padding: 0pt 0.5pt;
 +
    font-weight: bold;
 +
    font-size: 10pt;
 +
    text-align: right;
 +
    background-color: white;
 +
    }
 +
#bodyContent table.month a { background:none; padding:0 }
 +
.day-active { color:#cd0000 }
 +
.day-empty  { color:#000000 }
 +
 +
</style>
 +
</html>
 +
 +
 +
{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
 +
</table>
 +
 +
{| align="center"
 +
|-valign="top"
 +
|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=05}}
 +
|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=06}}
 +
|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=07}}
 +
|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=08}}
 +
|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=09}}
 +
|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=10}}
 +
|}
==Thursday 16 July 2009==
==Thursday 16 July 2009==
===Results===
===Results===

Revision as of 14:56, 24 July 2009

May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14] [http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23] [http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30] [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15] [http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents

Thursday 16 July 2009

Results

  • Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
Promoter Reporter Resistance/Plate Growth Observation
J23101 E0240 Cm no growth
J23101 J06702 Cm 21 colonies
J23102 E0240 Cm 3 colonies
J23102 J06702 Cm 8 colonies
J23103 E0240 Cm 2 colonies
J23103 J06702 Cm 23 colonies
J23104 E0240 Cm 9 colonies
J23104 J06702 Cm 12 colonies
J23105 E0240 Cm no growth
J23105 J06702 Cm 14 colonies
J23107 E0240 Cm 3 colonies
J23107 J06702 Cm 3 colonies
  • No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
    • Vial may have thawed out too much after cells were taken out for the electrocompetency procedure

Trentay 14:31, 16 July 2009 (UTC)

Craig and Clay

  • No growth was seen on either the kanamycin or chloramphenicol plates.
  • The sequence for the LIM1 gene was verified to be optimized for E. coli.

Tasks

Maria and Afton

  • Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
  • Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
  • Run an electrophoresis test on promoters and reporter Eo240
  • Pick colonies of promoters from transformation 100-107 (excluding 106)
  • Transformation of NEB 10 beta to make sure new stock is electrocompetent

Trentay 20:41, 16 July 2009 (UTC)

Craig and Clay

  • The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.

Wetlab

Maria and Afton

  • Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
  • Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
  • Run an electrophoresis test on promoters and reporter Eo240

Trentay 20:41, 16 July 2009 (UTC)

Retrieved from "http://2009.igem.org/Virginia_Commonwealth/16_July_2009"