Team:Calgary/26 May 2009

(Difference between revisions)

Revision as of 16:48, 28 July 2009

University of Calgary

NOTEBOOK PAGE INDEX Carol Emily Jamie Jeremy Kevin Vicki		MAY 26, 2009

		CAROL Descriptive title of what you're doing - no need for caps WIKI CODING HERE

		EMILY Isolation of LuxOD47E plasmids from pCR2.1-TOPO and PsB1AC3 vectors Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions. Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations Plasmid 260/280 260/230 Concentration [ng/μL] LuxOD47E Topo C1 1.88 2.21 409.5 LuxOD47E Topo C2 1.86 2.18 551.4 LuxOD47E BBK C1 2.04 5.01 551.4 LuxOD47E BBK C2 1.77 1.39 92.9

		JAMIE Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3 Sigma Genelute Plasmid Mini-prep kit was used. Standard manufacturer protocol used; elution in 50µL of ddH₂O. hi i love you

		JEREMY Descriptive title of what you're doing - no need for caps WIKI CODING HERE

		KEVIN Descriptive title of what you're doing - no need for caps WIKI CODING HERE

		VICKI Descriptive title of what you're doing - no need for caps WIKI CODING HERE

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-Descriptive title of what you're doing - no need for caps
+Isolation of LuxOD47E plasmids from pCR2.1-TOPO and PsB1AC3 vectors
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-WIKI CODING HERE
+Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
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+Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
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+Plasmid                        260/280                           260/230                    Concentration [ng/μL]
+LuxOD47E Topo C1    1.88                                 2.21                          409.5
+LuxOD47E Topo C2    1.86                                 2.18                          551.4
+LuxOD47E BBK C1    2.04                                 5.01                          551.4
+LuxOD47E BBK C2    1.77                                 1.39                          92.9
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