From 2009.igem.org
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Revision as of 17:08, 28 July 2009
University of Calgary
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CAROL
Descriptive title of what you're doing - no need for caps
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EMILY
Isolation of LuxOD47E plasmids from pCR2.1-TOPO and PsB1AC3 vectors
- Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
- Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
| Plasmid
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| 260/280
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| 230/280
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| Concentration [ng/μL]
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| LuxOD47E TOPO C1
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| 1.88
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| 2.21
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| 409.5
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| LuxOD47E TOPO C2
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| 1.86
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| 2.18
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| 551.4
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| LuxOD47E BBK C1
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| 2.04
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| 5.01
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| 551.4
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| LuxOD47E BBK C2
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| 1.77
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| 1.39
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| 92.9
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JAMIE
Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3
Sigma Genelute Plasmid Mini-prep kit was used. Standard manufacturer protocol used; elution in 50µL of ddH 2O.
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JEREMY
Descriptive title of what you're doing - no need for caps
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KEVIN
Descriptive title of what you're doing - no need for caps
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VICKI
Descriptive title of what you're doing - no need for caps
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"
