From 2009.igem.org
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| - | '''Plasmid''' '''260/280 230/280 Concentration''' [ng/μL]
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| - | <br>
| + | | Plasmid || 260/280 || 260/230 || Concentration [ng/μL] |
| - | LuxOD47E TOPO C1 1.81 2.21 409.5 | + | |- |
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| + | | LuxOD47E TOPO C1 || 1.88 || 2.21 || 409.5 |
| - | LuxOD47E TOPO C2 1.86 2.18 551.4 | + | |- |
| - | <Br>
| + | | LuxOD47E TOPO C2|| 1.86 || 2.18 ||551.4 |
| - | LuxOD47E BBK C1 2.04 5.01 551.4
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| - | <Br>
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| - | LuxOD47E BBK C2 1.77 1.39 92.9
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Revision as of 17:22, 28 July 2009
University of Calgary
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CAROL
Descriptive title of what you're doing - no need for caps
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EMILY
Isolation of LuxOD47E plasmids from pCR2.1-TOPO and PsB1AC3 vectors
- Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
- Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
| Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
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| LuxOD47E TOPO C1 | 1.88 | 2.21 | 409.5
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| LuxOD47E TOPO C2 | 1.86 | 2.18 | 551.4
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JAMIE
Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3
Sigma Genelute Plasmid Mini-prep kit was used. Standard manufacturer protocol used; elution in 50µL of ddH 2O.
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JEREMY
Descriptive title of what you're doing - no need for caps
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KEVIN
Descriptive title of what you're doing - no need for caps
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VICKI
Descriptive title of what you're doing - no need for caps
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"
