From 2009.igem.org
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| - | Isolation of LuxOD47E plasmids from pCR2.1-TOPO and PsB1AC3 vectors | + | Plasmid Isolation of <i>LuxOD47E</i> in pCR2.1 TOPO and psB1AC3 vectors |
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Revision as of 17:28, 28 July 2009
University of Calgary
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CAROL
Descriptive title of what you're doing - no need for caps
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EMILY
Plasmid Isolation of LuxOD47E in pCR2.1 TOPO and psB1AC3 vectors
- Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
- Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
| Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
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| LuxOD47E TOPO C1 | 1.88 | 2.21 | 409.5
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| LuxOD47E TOPO C2 | 1.86 | 2.18 | 551.4
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| LuxOD47E BBK C1 | 2.04 | 5.01 | 551.4
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| LuxOD47E BBK C2 | 1.77 | 1.39 | 92.9
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JAMIE
Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3
Sigma Genelute Plasmid Mini-prep kit was used. Standard manufacturer protocol used; elution in 50µL of ddH 2O.
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JEREMY
Descriptive title of what you're doing - no need for caps
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KEVIN
Descriptive title of what you're doing - no need for caps
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VICKI
Descriptive title of what you're doing - no need for caps
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