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| text-align: justify; | | text-align: justify; |
| } | | } |
| + | |
| + | .center { |
| + | text-align: center; |
| + | display: block; |
| + | margin-left: auto; |
| + | margin-right: auto; |
| + | } |
| + | |
| </style> | | </style> |
| | | |
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| <div id="leftmenu"> | | <div id="leftmenu"> |
| <td width="210" rowspan=30 bgcolor="#414141" valign="top"> | | <td width="210" rowspan=30 bgcolor="#414141" valign="top"> |
- | <br><center>
| |
| <br> | | <br> |
| + | <div class="heading">THIS MONTH</div> |
| + | <div class="desc"> |
| + | <center> |
| + | </html> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=05 | title=Team:Calgary}} |
| + | |} |
| + | <html> |
| + | </center> |
| + | </div> |
| <br> | | <br> |
- |
| |
- | <table>
| |
- | <tr>
| |
- | <td>
| |
- | <table class="month">
| |
- |
| |
- | <tr class="heading">
| |
- | <td colspan="7">May
| |
- | </td></tr>
| |
- | <tr class="dow">
| |
- | <td>M</td><td>T</td><td>W</td><td>T</td><td>F</td><td>S</td><td>S
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td>
| |
- | </td><td>
| |
- | </td><td>
| |
- | </td><td>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/1_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/1_May_2009" rel="nofollow"><span class="day-active">1</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/2_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/2_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">2</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/3_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/3_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">3</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/4_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/4_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">4</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/5_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/5_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">5</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/6_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/6_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">6</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/7_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/7_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">7</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/8_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/8_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">8</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/9_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/9_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">9</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/10_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/10_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">10</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/11_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/11_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">11</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/12_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/12_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">12</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/13_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/13_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">13</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/14_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/14_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">14</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/15_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/15_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">15</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/16_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/16_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">16</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/17_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/17_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">17</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/18_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/18_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">18</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/19_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/19_May_2009" rel="nofollow"><span class="day-active">19</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/20_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/20_May_2009" rel="nofollow"><span class="day-active">20</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/21_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/21_May_2009" rel="nofollow"><span class="day-active">21</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/22_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/22_May_2009" rel="nofollow"><span class="day-active">22</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/23_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/23_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">23</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/24_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/24_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">24</span></a>
| |
- | </td></tr>
| |
- | <tr>
| |
- | <td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/25_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/25_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">25</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/26_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/26_May_2009" rel="nofollow"><span class="day-active">26</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/27_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/27_May_2009" rel="nofollow"><span class="day-active">27</span></a>
| |
- | </td><td><a href="https://2009.igem.org/Team:Calgary/28_May_2009" class="external text" title="https://2009.igem.org/Team:Calgary/28_May_2009" rel="nofollow"><span class="day-active">28</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/29_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/29_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">29</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/30_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/30_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">30</span></a>
| |
- | </td><td><a href="https://2009.igem.org/wiki/index.php?title=Team:Calgary/31_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" class="external text" title="https://2009.igem.org/wiki/index.php?title=Team:Calgary/31_May_2009&preload=Team:Calgary/NotebookPreload&action=edit" rel="nofollow"><span class="day-empty">31</span></a>
| |
- | </td></tr></table>
| |
- | </td></tr></table>
| |
- | <p>
| |
- |
| |
- | </center>
| |
| <div class="heading">NOTEBOOK PAGE INDEX</div> | | <div class="heading">NOTEBOOK PAGE INDEX</div> |
| <div class="desc"> | | <div class="desc"> |
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| </div> | | </div> |
| </p></div> | | </p></div> |
| + | <br> |
| + | <br> |
| + | <div class="heading">CALENDAR</div> |
| + | <div class="desc"> |
| + | <center> |
| + | </html> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=05 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=06 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=07 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=08 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=09 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | {| style="background:transparent;" |
| + | |{{#calendar: query=preload=Team:Calgary/NotebookPreload | year=2009 | month=10 | title=Team:Calgary}} |
| + | |} |
| + | <br> |
| + | <html> |
| + | <center> |
| + | </desc> |
| </td> | | </td> |
| <td width="10" rowspan=30 bgcolor="#222222"> | | <td width="10" rowspan=30 bgcolor="#222222"> |
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| <br> | | <br> |
| <div class="heading"> | | <div class="heading"> |
- | MAY 27, 2009
| + | May 19, 2009 |
| </div> | | </div> |
| <br> | | <br> |
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| <br> | | <br> |
| <div class="heading"> | | <div class="heading"> |
- | Descriptive Title of What You're Doing | + | Descriptive Title of What You’re Doing |
| </div> | | </div> |
| <br> | | <br> |
| <div class="desc"> | | <div class="desc"> |
| </html> | | </html> |
- | WIKI CODING HERE: | + | WIKI CODING HERE |
- | | + | |
- | *bullet
| + | |
- | <br>
| + | |
- | <b>bold</b>
| + | |
- | <i>italics</i>
| + | |
- | <u>underline</u>
| + | |
- | <br>
| + | |
- | # Named link: [http://en.wikipedia.org Wikipedia]
| + | |
- | <br>
| + | |
- | link to books using ISBNs
| + | |
- | <br>
| + | |
- | [[Image:wiki.png]]
| + | |
- | will end up like:<br>
| + | |
- | [[Image:Thane4.png]]
| + | |
- | | + | |
| <html> | | <html> |
| </div> | | </div> |
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| </td> | | </td> |
| </tr> | | </tr> |
| + | |
| | | |
| <tr> | | <tr> |
|
CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
|
|
CHINMOYEE
Descriptive Title of What You're Doing
|
|
EMILY
Plasmid Isolation of LuxOD47E in pCR2.1 TOPO and psB1AC3 vectors
- Protocol: Used Sigma GenElute Plasmid Miniprep Kit (Sigma), lot # 048k6062, used as according to the manufacturers instructions.
- Results: Used Nanodrop 1000 Spectrophotometer read at 260 wavelength to determine DNA concentrations
Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
|
LuxOD47E TOPO C1 | 1.88 | 2.21 | 409.5
|
LuxOD47E TOPO C2 | 1.86 | 2.18 | 551.4
|
LuxOD47E BBK C1 | 2.04 | 5.01 | 551.4
|
LuxOD47E BBK C2 | 1.77 | 1.39 | 92.9
|
|
|
FAHD
Descriptive Title of What You're Doing
|
|
IMAN
Descriptive Title of What You're Doing
|
|
JAMIE
Plasmid Isolation of luxOU in pCR-Blunt II-TOPO and psB1AC3
Sigma Genelute Plasmid Mini-prep kit (lot # 048k6062)as used. Standard manufacturer protocol used; elution in 50µL of ddH 2O.
Plasmid sample |
260/280 |
260/230 |
Concentration(ng/µL) |
TOP10 luxOU C1 |
1.91 |
2.58 |
365.6 |
TOP10 luxOU C2 |
1.90 |
2.52 |
287.2 |
BBK luxOU C1 |
1.87 |
2.22 |
534.2 |
BBK luxOU C2 |
1.92 |
2.24 |
715.2 |
|
|
JEREMY
Descriptive Title of What You're Doing
|
|
KATIE
Descriptive Title of What You're Doing
|
|
KEVIN
Plasmid Isolation of Pqrr4 for Reporter circuit
The Pqrr4 was isolated from TOPO T/A and biobrick AC (pSB1AC3) using Sigma’s Genelute Plasmid Mini-prep kit (sigma) according to the specifications of the manufacturer. Nanodrop utility along with the Spectrophotometer was used to measure the concentration and purity of the isolated plasmid. The wavelength was set at 260nm.
Plasmid | 260/280 | 260/230 | Concentration [ng/μL]
|
Pqrr4 TOPO Blue tube | 1.81 | 2.24 | 181
|
Pqrr4 TOPO Pink tube | 1.81 | 2.02 | 231.3
|
Pqrr4 BBK Green tube | 1.76 | 1.66 | 51.2
|
Pqrr4 BBK Pink tube | 1.85 | 1.45 | 60.6
|
*The two TOPO vectors were from the same colony, thus labelled "Green tube" instead of C1. The two BBK plasmids were also from same colony, so same label applies.
|
|
MANDY
Descriptive Title of What You're Doing
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
PRIMA
Descriptive Title of What You're Doing
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
VICKI
Descriptive Title of What You're Doing
|