Team:Osaka/Brainstorming2

From 2009.igem.org

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=07/10/2009 Paint and Illumination=
 
Brainstorming with one brain that belongs to nori
Brainstorming with one brain that belongs to nori
==Operation==
==Operation==
-
===1)Cell to Cell communication (Quorum sensing)===
+
===Cell to Cell communication (Quorum sensing)===
*orthogonal cell-cell signaling will be required for our ART painting
*orthogonal cell-cell signaling will be required for our ART painting
Existing systems->
Existing systems->
-
*LuxI-LuxR
+
*LuxI-LuxR  
*LasI-LasR
*LasI-LasR
*ciniI-cinR
*ciniI-cinR
*RhII-RhIR
*RhII-RhIR
 +
 +
*Evaluate diffuse of signals - PoPs, RiPs and Degradation
 +
 +
===Timer or counter(optional addon)===
 +
*well known genetic circuit
 +
*light or sound induced?
 +
 +
==Output -pattern and painting- ==
 +
===patterning===
 +
we have to control the motility of E.coli or salmonella on soft-agar plate
 +
 +
===Stop rotation of flagella motor===
 +
*use molecular clutch (iGEM08 Imperial college) [[http://partsregistry.org/partsdb/get_part.cgi?part=BBa_K143032]]
 +
->However, this parts was characterized only in butilus.
 +
 +
===Stop viased random walk===
 +
express che?
 +
 +
===Stop flagellar assembly===
 +
*use the strain that lacks flagella gene. But Inactivating auto inducer will be needed!
 +
 +
 +
==Art Challange==
 +
*no team had done this theme. Ethics and environmental issue was emphasized.

Latest revision as of 20:11, 29 July 2009

Brainstorming with one brain that belongs to nori

Contents

Operation

Cell to Cell communication (Quorum sensing)

  • orthogonal cell-cell signaling will be required for our ART painting

Existing systems->

  • LuxI-LuxR
  • LasI-LasR
  • ciniI-cinR
  • RhII-RhIR
  • Evaluate diffuse of signals - PoPs, RiPs and Degradation

Timer or counter(optional addon)

  • well known genetic circuit
  • light or sound induced?

Output -pattern and painting-

patterning

we have to control the motility of E.coli or salmonella on soft-agar plate

Stop rotation of flagella motor

->However, this parts was characterized only in butilus.

Stop viased random walk

express che?

Stop flagellar assembly

  • use the strain that lacks flagella gene. But Inactivating auto inducer will be needed!


Art Challange

  • no team had done this theme. Ethics and environmental issue was emphasized.