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Team:Calgary/11 June 2009
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| - | + | Plasmid Isolation of <i>LuxCDABE</i> (luciferase) | |
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| - | + | The LuxCDABE is being isolated by Caron Chan, but she needed some hands on this, and I was willing to help. LuxCDABE was isolated with Quigen's EndoFree Plasmid Maxi (quigen) according to the specification of the provider. A different kit than the one used in previous experiments was used because the LuxCDABE is very big in size (6kb), and the Minprep would not be capable of isolating the plasmid at a high enough concentration. | |
| + | Despite of the use of Maxiprep, however, the spectrophotometer seemed to have not picked up any plasmid in the isolated sample, as the purity and concentration were below acceptable. Another Maxiprep may be needed to be done. | ||
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Revision as of 22:57, 30 July 2009
UNIVERSITY OF CALGARY
