From 2009.igem.org
(Difference between revisions)
|
|
| Line 357: |
Line 357: |
| | <br> | | <br> |
| | <div class="heading"> | | <div class="heading"> |
| - | Descriptive Title of What You're Doing
| + | Plasmid Isolation and Restriction Digest of cl lambda and PQ-OU |
| | </div> | | </div> |
| | <br> | | <br> |
| | <div class="desc"> | | <div class="desc"> |
| | </html> | | </html> |
| - | WIKI CODING HERE
| + | Plasmid was isolated from overnight cultures of cl lambda (two colonies – four tubes each) and PQ-B-R-OU-B in AK (4 colonies) using the QIAprep Spin MiniPrep Kit (QIAGEN). Plasmid purity and concentration were measured using the NanoDrop Spectrophotometer. The four tubes per colony of cl lambda were pooled and then vacufuged for two hours to increase the concentration of plasmid. RD was then set up with XbaI and PstI for 2 hours at 37 degrees for all the isolated and pooled plasmid. |
| | + | <br> |
| | + | <br> |
| | + | Sequencing results of LuxPQ-B0015-R0040-LuxOU-B0015 in psB1AC3 came back and the construct appears to be in the plasmid! Now we will be able to clone the PQ-OU construct into the Surrette vector and eventually start testing the promoter library. |
| | + | <br> |
| | + | <br> |
| | + | T-shirt companies are starting to be contacted concerning the awesome T-shirts that the U of C’s iGEM team will sport at the iGEM jamboree. |
| | + | |
| | | | |
| | <html> | | <html> |
Revision as of 23:13, 4 August 2009
University of Calgary
|
|
CAROL
Modelling Meeting
- No lab experiments performed today (have to re-consider methods) - no colonies were found from the transformation that occured yesterday morning.
- For the modelling meeting, the following points were discussed:
1. A better organization of our work is required. We have both characterization and simulation from the mathematical team, but the work seems unlinked and not organized. Will have a more organized report for next week.
2. Greater detail was explained to us by Thane Kubik regarding the biochemical details that are behind the signalling pathway.
3. We need to start thinking about how we are going to present our two modelling system that we have created.
4. We will meet again on thursday and Afshin will give us a better overview of membrane computing.
|
|
|
CHINMOYEE
I'm Back
Today we had a really great and important modelling meeting. We looked at the biochemistry of the circuit and gained new insight on different concept concerning the circuits.
Iman showed us the visual representations he created .
A few pictures for the APEGGA article was taken and the final article was revised.
I revised the math modelling summary and sent it out to everyone. The simbiology simulations were also revised . New reactions were added to the old file.
Researched information on GFP and thought about how that individual circuit/ mutant circuit could be characterized .
Learned how to update on wiki.
New Jobs to do :
Prepare for the presentation on Friday : 12 min
Gauntlet article due Friday
Try to make an easier interface on simbiology ( eg . changing the initial concentration of ...)
Think about flow/content of Jambouree presentation :5 min content
|
|
|
EMILY
Descriptive Title of What You're Doing
|
|
|
FAHD
Marketing for August 4th
Today, I put my energy towards marketing our project. I made preparations for our 2009 iGEM fundraising Bake Sale which will be held tomorrow (Wednesday August 5th 2009).
I also worked on some grant stream applications such as t=The Enbridge Pipeline Inc. Community Support Grants and also contacted some Oil & Gas companies. The following is the list of companies I contacted today:
1)Mackenzie Aborginal Corporation (MAC)
2) KMC Mining
3) Imperial Oil Resources.
|
|
|
IMAN
Descriptive Title of What You're Doing
|
|
|
JAMIE
Descriptive Title of What You're Doing
|
|
|
JEREMY
Plasmid Isolation and Restriction Digest of cl lambda and PQ-OU
Plasmid was isolated from overnight cultures of cl lambda (two colonies – four tubes each) and PQ-B-R-OU-B in AK (4 colonies) using the QIAprep Spin MiniPrep Kit (QIAGEN). Plasmid purity and concentration were measured using the NanoDrop Spectrophotometer. The four tubes per colony of cl lambda were pooled and then vacufuged for two hours to increase the concentration of plasmid. RD was then set up with XbaI and PstI for 2 hours at 37 degrees for all the isolated and pooled plasmid.
Sequencing results of LuxPQ-B0015-R0040-LuxOU-B0015 in psB1AC3 came back and the construct appears to be in the plasmid! Now we will be able to clone the PQ-OU construct into the Surrette vector and eventually start testing the promoter library.
T-shirt companies are starting to be contacted concerning the awesome T-shirts that the U of C’s iGEM team will sport at the iGEM jamboree.
|
|
|
KATIE
Descriptive Title of What You're Doing
|
|
|
KEVIN
Preparation for Plasmid switch of Pqrr4+I13500
Pqrr4+I13500(RBS+GFP) was constructed to verify the Mutants; however, we realized that both of our parts were in high copy plasmids, which a single cell can take one of. Thus the smaller Pqrr4+I13500 circuit is chosen to be plasmid switched into another vector (pSB2K3), which was obtained from the Q04510 (inverter). Today, both the Pqrr4+13500 and pSB2K3 were cut at xbaI+PstI via restriction digest, and it is going to be left in the waterbath(37˚C) overnight.
Preparation for Plasmid switch of Pqrr4+I13500
|
|
|
MANDY
Descriptive Title of What You're Doing
|
|
|
PATRICK
Descriptive Title of What You're Doing
|
|
|
PRIMA
Descriptive Title of What You're Doing
|
|
|
STEFAN
Descriptive Title of What You're Doing
|
|
|
VICKI
Descriptive Title of What You're Doing
WIKI CODING HERE
|
"
