Virginia Commonwealth/30 July 2009

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Revision as of 14:01, 5 August 2009

May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14] [http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23] [http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30] [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15] [http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents

Thursday 30 July 2009

Results

Maria and Afton

  • The overnight culture grew, however, no RFP was expressed (I1352)

Trentay 14:26, 31 July 2009 (UTC)

Tasks

Maria and Afton

  • Make Cryogenic Stocks of part I1352 (2 vials)
  • Make another overnight culture from the previous culture to see if it will express RFP
  • Miniprep I1352
  • Take spectrophotometry measurements of I1352 and pSB4C5
  • Digest and Ligate I1352 and pSB4C5
  • Run a Gel Electrophoresis on pSB4C5 and I1352 using the Hyper Ladder and the 100bp ladder
  • Transform parts into NEB 10 beta

Trentay 14:26, 31 July 2009 (UTC)

Wetlab

Maria and Afton

  • Cryogenic stocks were made
  • Overnight culture of I1352 was grown up in 5mL of LB+AMP
  • 2 samples of 1.5mL of I1352 was Miniprepped
  • Spectophotometry measurements were taken
    • The two samples were combined into one tube before measurements were taken
    • pSB4C5 that was miniprepped on July 27, 2009
  • Digestion was done
    • Only the restriction enzymes EcoRI-HF and Pstl were used to cut the separate the backbone from the promter and RET
  • Ligation was done
    • for the Ligation of I1352 with pSB4C5, 3 microliters was added of each digestion to the Ligation sample
    • for the Ligation of I1352 with itself, 6 microliters of the Digestion was addes to the Ligation sample
  • Gel Electrophoresis was run at 120v for 45 min and DNA bands appeared for both samples
  • Transformation was done on the two ligated samples

Trentay 15:10, 31 July 2009 (UTC)

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