Virginia Commonwealth/5 June 2009
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==Friday 05 June 2009== | ==Friday 05 June 2009== | ||
===Results=== | ===Results=== | ||
+ | *Observations on the Colonies | ||
+ | |||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Part No.!! Plate Type !! Colonies !! Other Observations !! | ||
+ | |- | ||
+ | ! J13002 | ||
+ | | Amp || 10s of colonies || n/a || | ||
+ | |- | ||
+ | ! PSB 1C3 | ||
+ | | Cm || 10s of colonies || Pinkish in color (RFP expression) || | ||
+ | |- | ||
+ | ! R0040 | ||
+ | | Amp || 10s of colonies || n/a || | ||
+ | |- | ||
+ | ! E1010 | ||
+ | | Kan || 100s of colonies || n/a || | ||
+ | |- | ||
+ | ! PSB 4C5 | ||
+ | | Cm || about 9 colonies || n/a || | ||
+ | |- | ||
+ | ! E0840 | ||
+ | | Amp ||10s of colonies || n/a || | ||
+ | |} | ||
+ | |||
+ | *Observations on the Controls | ||
+ | |||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Control !! Plate Type !! Observation !! | ||
+ | |- | ||
+ | ! NEB10β (+) | ||
+ | | LB || lawn || | ||
+ | |- | ||
+ | ! NEB10β (-) | ||
+ | | Cm || no growth || | ||
+ | |- | ||
+ | ! NEB10β (-) | ||
+ | | Amp || no growth || | ||
+ | |- | ||
+ | ! NEB10β (-) | ||
+ | | Kan || no growth || | ||
+ | |- | ||
+ | ! DB3.1 (-) | ||
+ | | Cm || no growth || | ||
+ | |- | ||
+ | ! DB3.1(+) | ||
+ | | LB ||lawn || | ||
+ | |} | ||
+ | |||
+ | [[User:MandM|MandM]] 16:14, 7 June 2009 (UTC) | ||
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
+ | *Literature discussion | ||
+ | *Genetic engineering work flow discussion | ||
---- | ---- | ||
- | |||
====Wetlab==== | ====Wetlab==== | ||
*Pick transformed colonies and culture overnight (5mL) | *Pick transformed colonies and culture overnight (5mL) | ||
+ | *Introduction to media making | ||
+ | ---- |
Latest revision as of 14:12, 5 August 2009
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Contents |
Friday 05 June 2009
Results
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
J13002 | Amp | 10s of colonies | n/a | |
PSB 1C3 | Cm | 10s of colonies | Pinkish in color (RFP expression) | |
R0040 | Amp | 10s of colonies | n/a | |
E1010 | Kan | 100s of colonies | n/a | |
PSB 4C5 | Cm | about 9 colonies | n/a | |
E0840 | Amp | 10s of colonies | n/a |
- Observations on the Controls
Control | Plate Type | Observation | |
---|---|---|---|
NEB10β (+) | LB | lawn | |
NEB10β (-) | Cm | no growth | |
NEB10β (-) | Amp | no growth | |
NEB10β (-) | Kan | no growth | |
DB3.1 (-) | Cm | no growth | |
DB3.1(+) | LB | lawn |
MandM 16:14, 7 June 2009 (UTC)
Tasks
- Literature discussion
- Genetic engineering work flow discussion
Wetlab
- Pick transformed colonies and culture overnight (5mL)
- Introduction to media making