Team:Valencia/Notebook/August

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(New page: __NOTOC__ <div style=" width:94%; margin: 0 auto;"> <!-- ESCRIBIR AQUI --> ===August 1st=== - We carried out the experiment with [[Team:Valencia/Notebook/Protocols#YPKAc Medium | YPKAc M...)
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| 13||[[Team:Valencia/Notebook/April#April 7th to April 18th | 14]] || [[Team:Valencia/Notebook/April#April 7th to April 18th |15]] || [[Team:Valencia/Notebook/April#April 7th to April 18th |16]] || [[Team:Valencia/Notebook/April#April 7th to April 18th |17]] || [[Team:Valencia/Notebook/April#April 7th to April 18th |18]] || 19 ||
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| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;[[Team:Valencia/Notebook/July#July 1st|1]] || &nbsp;[[Team:Valencia/Notebook/July #July 2nd|2]] || &nbsp;3 || &nbsp;[[Team:Valencia/Notebook/July #July 4th|4]] || &nbsp;5 ||
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| &nbsp;6||&nbsp;[[Team:Valencia/Notebook/July#July 7th|7]] || &nbsp;[[Team:Valencia/Notebook/July#July 8th|8]] || &nbsp;&nbsp;[[Team:Valencia/Notebook/July#July 9th|9]] || [[Team:Valencia/Notebook/July#July 10th|10]] || [[Team:Valencia/Notebook/July#July 11th|11]] || 12 ||
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| 13 ||[[Team:Valencia/Notebook/July#July 14th|14]] || [[Team:Valencia/Notebook/July#July 15th|15]] || &nbsp;[[Team:Valencia/Notebook/July#July 16th|16]] || [[Team:Valencia/Notebook/July#July 17th|17]] || [[Team:Valencia/Notebook/July#July 18th|18]] || 19 ||
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| 20||[[Team:Valencia/Notebook/July#July 21st|21]] || [[Team:Valencia/Notebook/July#July 22nd|22]] || &nbsp;[[Team:Valencia/Notebook/July#July 23rd|23]] || [[Team:Valencia/Notebook/July#July 24th|24]] || [[Team:Valencia/Notebook/July#July 25th|25]] || [[Team:Valencia/Notebook/July#July 26th|26]] ||
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| 27||[[Team:Valencia/Notebook/July#July 28th|28]]|| [[Team:Valencia/Notebook/July#July 29th|29]] || &nbsp;[[Team:Valencia/Notebook/July#July 30th|30]] || [[Team:Valencia/Notebook/July#July 31st|31]] || <Font Color="#c0c0c0">&nbsp;&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
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| &nbsp;3 ||&nbsp;4 || &nbsp;[[Team:Valencia/Notebook/August#August 5th|5]] || &nbsp;&nbsp;[[Team:Valencia/Notebook/August#August 6th|6]] || &nbsp;[[Team:Valencia/Notebook/August#August 7th|7]] || &nbsp;[[Team:Valencia/Notebook/August#August 8th|8]] || &nbsp;[[Team:Valencia/Notebook/August#August 9th|9]]
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| 10 || [[Team:Valencia/Notebook/August#August 11th|11]] || [[Team:Valencia/Notebook/August#August 12th|12]] || &nbsp;[[Team:Valencia/Notebook/August#August 13th|13]]|| [[Team:Valencia/Notebook/August#August 14th|14]]|| [[Team:Valencia/Notebook/August#August 15th|15]] || 16
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|17|| [[Team:Valencia/Notebook/August#August 18th|18]] || 19 || &nbsp;20 || [[Team:Valencia/Notebook/August#August 21st|21]] || 22 || [[Team:Valencia/Notebook/August#August 23rd|23]]
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| 24||[[Team:Valencia/Notebook/August#August 25th|25]] || 26 || &nbsp;[[Team:Valencia/Notebook/August#August 27th | 27]] || 28 || [[Team:Valencia/Notebook/August#August 29th | 29 ]] || [[Team:Valencia/Notebook/August#August 30th | 30]]
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| <Font Color="#c0c0c0">31</Font>||&nbsp;1 || &nbsp;[[Team:Valencia/Notebook/September #September 2nd|2]] || &nbsp;&nbsp;[[Team:Valencia/Notebook/September #September 3rd|3]] || &nbsp;4 || &nbsp;[[Team:Valencia/Notebook/September #September 5th|5]] || &nbsp;6||
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| &nbsp;7||&nbsp;[[Team:Valencia/Notebook/September #September 8th|8]] || &nbsp;[[Team:Valencia/Notebook/September #September 9th|9]] || &nbsp;10 || [[Team:Valencia/Notebook/September #September 11th|11]] || 12 || 13 || 14
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| [[Team:Valencia/Notebook/October#October 13th |13]] || [[Team:Valencia/Notebook/October#October 14th |14]]  || &nbsp;15 || 16 || [[Team:Valencia/Notebook/October#October 17th |17]] || 18 || 19
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| [[Team:Valencia/Notebook/October#October 20th |20]] || 21 || &nbsp;22 || [[Team:Valencia/Notebook/October#October 23rd |23]]  || [[Team:Valencia/Notebook/October#October 24th |24]]  || 25 || 26
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| &nbsp;3 || &nbsp;4 || &nbsp;&nbsp;5 || &nbsp;6 || &nbsp;7|| &nbsp;[[Team:Valencia/Notebook/November#8 & 9/10/08|8]] || &nbsp;[[Team:Valencia/Notebook/November#8 & 9/10/08|9]]
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Revision as of 14:35, 5 August 2009


Home Team Project Human Practices Notebook News

April
Mo Tu We Th Fr Sa Su
30 31  1 2  3  4  5
 6  7  8 9 10 11 12
13 14 15 16 17 18 19
2021 22 23 24 25 26
2728 29  30  1  2  3
May
Mo Tu We Th Fr Sa Su
27 28 29 30  1  2  3
 4 5  6 7  8  9 10
1112 13 14 15 16 17
1819 20 21 22 23 24
2526 27  28 29 30 31
June
Mo Tu We Th Fr Sa Su
 1 2  3   4  5  6  7
 8  9 10  11 12 13 14
1516 17  18 19 20 21
2223 24  25 26 27 28
2930  1   2  3  4  5
July
Mo Tu We Th Fr Sa Su
29 30  1  2  3  4  5
 6 7  8   9 10 11 12
13 14 15  16 17 18 19
2021 22  23 24 25 26
2728 29  30 31   1  2
August
Mo Tu We Th Fr Sa Su
27 28 29  30 31  1  2
 3  4  5   6  7  8  9
10 11 12  13 14 15 16
17 18 19  20 21 22 23
2425 26   27 28 29 30
311 2 3 4 5 6
September
Mo Tu We Th Fr Sa Su
31 1  2   3  4  5  6
 7 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30    1   2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30

August 1st

- We carried out the experiment with YPKAc Medium . We were just characterizing yeast growth in this medium. In this medium containing acetate, yeast is supposed to only breathe rather than fermenting.

-Team meeting: Checking project progress and planning the following experiments.

August 5th

- Experiment carried out:
       SP medium.
      Four strains.
      No galactose activation.

August 6th

-Trying to find out the best moment for galactose activation....
       YPKAc Medium .
      Galactose activation at two, three and four hours.
      O.D. measured at each time.

August 7th

-We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment:
       SP medium.
      Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating.
      Strain UCP+ just with galactose induction.

August 8th

-We prepared lab material and decided the following experiment we would carry out.

August 9th

-We carried out the experiment changing some variables:
       SP medium.
      Four strains.
      Inoculation at O.D. around 0.2
      Galactose induction 5 hours after inoculating.

August 11th

-The thermocouples inside the calorimeters had been always placed in the upper part, having no contact with the liquid because of contamination reasons. Our engineers team mates suggested it would be better for the thermocouples to be submerged in the liquid. We carried out an experiment to see if there are significant differences:
      Four calorimeters.
      Water inside.
      Two submerged, two just in contact with the air inside the calorimeter.


-Team meeting: Checking project progress.

August 12th

-We had growing problems. Apparently, our cultures were too old...
-We repeated the experiment from yesterday, changing some variables:
      We incremented the shaking.
      We incremented the tilt.
We obtained surprising results, our calorimeters heated the water up!

August 13th

-Still having growing problems.
-We repeated the experiment from yesterday. This time with no so much tilt.

August 14th

-We carried out an experiment both to charazterize yeast growth and to check if the strains behave as they should, since those expresing thermogenine should grow at a lower rate.
      Four strains in Erlenmeyer flash inside the 30ºC shaking stove.
      Galactose induction at O.D. 0.2
      O.D. measurements every one and a half hours.
      250 rpm.

August 15th

-We charactarized yeast growing in the calorimeters, in the same conditions as yesterday.
After the last measurement (9 hours), we induced a second time with 5 ml galactose.

August 18th

-We repeated exactly the same experiment as August 15th, in order to see if we could obtain the same good results.
Initial temperature was lower because we were not able to completely control it.

August 21st

-We were trying to determine the conditions to obtain temperature increase as we did on August 15th:
      Four strains.
      Initial O.D. 0.2
      Galactose at 3 hours.
      250 rpm

August 23rd

-We repeated the experiment from yesterday. Same conditions.

August 25th

-Trying to determine whether we would obtain different results with YPKAc Medium. Experiment:
      175 Δ strain.
      Initial OD 0.2
      Two flasks with SP medium, two flasks with YPKAc.
      For each medium, one of them with galactose induction.

August 27th

-We carried out the following experiment:
      Four strains in LCCs with SP medium.
      Galactose induction at the beginning. Initial O.D. 0,6
      275 rpm.
We had to stop it because one of the LCC was broken.

August 28th

Joaquina's birthday!!

August 29th

-We carried out the experiment again:
      Four strains in LCCs with SP medium.
      Galactose induction at the beginning. Initial O.D. 0,6
      275 rpm.

August 30th

One of the strains was increasing its culture temperature. We decided to leave the experiment a couple more hours.


Back to July    |    August    |    Go to September

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