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Virginia Commonwealth/26 June 2009
From 2009.igem.org
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==Friday 26 June 2009== | ==Friday 26 June 2009== | ||
===Results=== | ===Results=== | ||
| - | * | + | * Plate growing pSB3K3 plasmid grew well. There was no fluorescence expressed. |
| - | * | + | * Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well. There was no fluorescence expressed. |
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
| + | |||
| + | * Pick colonies and grow overnight. | ||
| + | * Confirm the growing colonies using GFP and RFP reporter. | ||
| + | * Order DNA and IPTG | ||
| + | * Additional promoters were designed. These possessed an up element that is proven to bind with the alpha subunit of RNAP by multiple sources. | ||
---- | ---- | ||
| - | |||
| - | |||
| - | |||
====Wetlab==== | ====Wetlab==== | ||
| - | *IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. - | + | * IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. - |
| - | * | + | * IPTG stock (5uL) was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP). After several hours no fluorescence was expressed. 3 mL LB+KAN was added to each culture so that the cells could re-enter log phase growth. 3 uL IPTG was also added to each culture to maintain concentration. |
Latest revision as of 19:40, 5 August 2009
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Contents |
Friday 26 June 2009
Results
- Plate growing pSB3K3 plasmid grew well. There was no fluorescence expressed.
- Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well. There was no fluorescence expressed.
Tasks
- Pick colonies and grow overnight.
- Confirm the growing colonies using GFP and RFP reporter.
- Order DNA and IPTG
- Additional promoters were designed. These possessed an up element that is proven to bind with the alpha subunit of RNAP by multiple sources.
Wetlab
- IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. -
- IPTG stock (5uL) was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP). After several hours no fluorescence was expressed. 3 mL LB+KAN was added to each culture so that the cells could re-enter log phase growth. 3 uL IPTG was also added to each culture to maintain concentration.
