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Virginia Commonwealth/28 June 2009
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==Sunday 28 June 2009== | ==Sunday 28 June 2009== | ||
===Results=== | ===Results=== | ||
| - | * | + | * n/a |
| - | + | ||
---- | ---- | ||
===Tasks=== | ===Tasks=== | ||
| + | * Today we need to plate the cells containing the PSB3K3 plasmid and the death gene P1010. | ||
---- | ---- | ||
| - | |||
| - | |||
====Wetlab==== | ====Wetlab==== | ||
| - | * | + | * We plated NEB10β with the PSB3K3 plasmid. There were no major hitches in the steps leading to the incubation. (Note: The cells used were marked with having 50 microliters already removed.) |
| - | * | + | * However, when we went to incubate the plates before plating the E. Coli we discovered two unidentified fungi growing on the plates. One was a giant black colony and the other was a giant white colony. We are unsure of the kind of fungi or where they come from. Since these were our only supply of LB plates and we need to plate controls, we used plates from the same stack. We did, however, place these plates under UV light in the sterile hood for about 5 minutes and then they were placed back in the incubator. |
| + | * The controls for the experiment are: | ||
| + | ** E. Coli Cells on LB (+) | ||
| + | ** E. Coli Cells on LB with KAN (-) | ||
| + | ** E. Coli Cells Zapped on LB (+) | ||
| + | * The notebook was updated on page 16 by Afton. | ||
| + | [[User:MandM|MandM]] 23:37, 28 June 2009 (UTC) | ||
Latest revision as of 19:43, 5 August 2009
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Contents |
Sunday 28 June 2009
Results
- n/a
Tasks
- Today we need to plate the cells containing the PSB3K3 plasmid and the death gene P1010.
Wetlab
- We plated NEB10β with the PSB3K3 plasmid. There were no major hitches in the steps leading to the incubation. (Note: The cells used were marked with having 50 microliters already removed.)
- However, when we went to incubate the plates before plating the E. Coli we discovered two unidentified fungi growing on the plates. One was a giant black colony and the other was a giant white colony. We are unsure of the kind of fungi or where they come from. Since these were our only supply of LB plates and we need to plate controls, we used plates from the same stack. We did, however, place these plates under UV light in the sterile hood for about 5 minutes and then they were placed back in the incubator.
- The controls for the experiment are:
- E. Coli Cells on LB (+)
- E. Coli Cells on LB with KAN (-)
- E. Coli Cells Zapped on LB (+)
- The notebook was updated on page 16 by Afton.
MandM 23:37, 28 June 2009 (UTC)
