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| - | Descriptive Title of What You're Doing
| + | Continuation of August 5<sup>th</sup> |
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| - | WIKI CODING HERE
| + | DNA replication progress: I need to get the polymerase to move along the strand of DNA, the leading strand is much easier since it actually does not have to detect anything and just rez the nucleotides as it moves down the strand, but the RNA primers will no longer be able to be physical so I will have to use another method. |
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| | + | I made slides and edited video clips and snapshots that were taken yesterday for second life presentation tomorrow for the virtual lab and the synthetic kingdom and the results so far for both domains. There is one video for each as well as some explanatory slides. |
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| | + | Also, some changes were made to note cards and substrings for the construction station since they were initially divided with back slashes and are now divided by spaces, which are not recognized as the note card reader so the substrings had to be changed. Another issue I discovered was that in order to properly secure one section of the DNA extraction activity I will require permission to modify the script in the cartridge since the centrifuge is not unlocked properly otherwise. |
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CAROL
Modelling Meeting and Presentation Discussions
- For the modelling meeting, we mainly discussed about what both teams need in terms of lab results, so that we can deligate different tasks for different people
- In preparations for tomorrow's lab formal meeting, we have prepared and deligated different slides and points that we want to discuss for tomorrow.
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CHINMOYEE
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EMILY
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FAHD
Another Day in Marketing and Ethics
Today, I worked on the Ethics and Marketing portion of our project.
I started my day of with doing research on some pharmaceutical and oil & gas companies and e-mailing him them our sponsorship package. I also prepared for our tomorrow’s presentation meeting. I also setup a meeting (in-studio interview) with our University Community Radio Station (CJSW 90.9) for next week.
For Ethics, I prepared for our ethics presentation for our tomorrow’s presentations meeting. I also explored Second Life so I can get more ideas of how to hold a conference in the online virtual world.
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IMAN
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JAMIE
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JEREMY
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KATIE
Continuation of August 5th
DNA replication progress: I need to get the polymerase to move along the strand of DNA, the leading strand is much easier since it actually does not have to detect anything and just rez the nucleotides as it moves down the strand, but the RNA primers will no longer be able to be physical so I will have to use another method.
I made slides and edited video clips and snapshots that were taken yesterday for second life presentation tomorrow for the virtual lab and the synthetic kingdom and the results so far for both domains. There is one video for each as well as some explanatory slides.
Also, some changes were made to note cards and substrings for the construction station since they were initially divided with back slashes and are now divided by spaces, which are not recognized as the note card reader so the substrings had to be changed. Another issue I discovered was that in order to properly secure one section of the DNA extraction activity I will require permission to modify the script in the cartridge since the centrifuge is not unlocked properly otherwise.
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KEVIN
1. Sequencing
The construct Pqrr4+B0034+K082003 (GFP:LVA) has been sent to for sequencing with Pqrr4 F/R primers to the University of Calgary DNA Sequencing Facility (University Core DNA Services, Calgary, Alberta, Canada). It has been submitted before 1pm, so we will be able to get the results tomorrow.
2. Overnight cultures
Overnight cultures of Pqrr4+I13500 were grown from the single colonies that were transformed yesterday. These are required for plasmid isolation of the circuit, which will then be verified via Restriction digest.
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MANDY
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PATRICK
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PRIMA
More sponsor/media/lab
I spent some time looking up and reading out aiiA, B0015 and B0034 and then Jeremy spent some time explaining restriction sites, primers, resistance, steps of construction and where my part fits into the system.
Next, I worked with Fahd to make a list of new companies we could contact for sponsorship but we'll divide them up tomorrow. I have started to research a few that i thought I'd like to contact. I also looked up the contact info for Utoday and I'll be contacting them tomorrow. I sent out the last few newsletters to companies and emailed our Communications/media adviser to discuss few issues about promoting iGEM.
Then the marketing, ethics and outreach team worked together to make the slides for tomorrow's formal presentation! We discussed the important issues, edited, and divided up the different sections among different group members.
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STEFAN
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VICKI
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