Team:Aberdeen Scotland/parameters/invest 1

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<td>LacI repressor dissociation constant</td>
<td>LacI repressor dissociation constant</td>
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<td>K<sub>IPTG</sub></td>
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<td>1.3 [µM]</td>
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<td>522 molecules</td>
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<td>IPTG-LacI repressor dissociation constant</td>
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<td>K<sub>tetR</sub></td>
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<td>179 [pM]</td>
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<td>0.07 molecules</td>
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<td>TetR repressor dissociation constant</td>
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<td>K<sub>CI</sub></td>
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<td>8 [pM] OR 50 [nM]</td>
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<td> 0.003 molecules OR 20 molecules</td>
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<td>CI repressor dissociation constant</td>
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<td>K<sub>AHL</sub></td>
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<td>0.09 - 1 [µM]</td>
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<td>402 molecules</td>
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<td>AHL-LuxR activator dissociation constant</td>
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Revision as of 10:31, 7 August 2009

University of Aberdeen iGEM 2009


Dissociation Constants

Introduction

Our model uses hill kinetics; we have three repression hill functions of the form:

Dissociation Constants Eq 1.gif

It also has one activation hill function of the form:

Dissociation Constants Eq 2.gif

And one repression / induction hill function of the form

Dissociation Constants Eq 3.gif
Dissociation Constants Eq 4.gif
Dissociation Constants Eq 5.gif


Parameter Value Value (molecules per cell) Description
KLacI 0.1 - 1 [pM] OR 800 [nM] 0.00004-0.0004 molecules OR 322 molecules LacI repressor dissociation constant
KIPTG 1.3 [µM] 522 molecules IPTG-LacI repressor dissociation constant
KtetR 179 [pM] 0.07 molecules TetR repressor dissociation constant
KCI 8 [pM] OR 50 [nM] 0.003 molecules OR 20 molecules CI repressor dissociation constant
KAHL 0.09 - 1 [µM] 402 molecules AHL-LuxR activator dissociation constant


 

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