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Team:Calgary/7 August 2009
From 2009.igem.org
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The <i>Pqrr4</i>+I13500 isolated plasmid was digested with XbaI and PstI enzymes, then ran on 1% gel at 100V. The following gel was obtained. | The <i>Pqrr4</i>+I13500 isolated plasmid was digested with XbaI and PstI enzymes, then ran on 1% gel at 100V. The following gel was obtained. | ||
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[[Image:2009.08.07.Pqrr4+I13500 pSB2k3.png|500px]] | [[Image:2009.08.07.Pqrr4+I13500 pSB2k3.png|500px]] | ||
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As one can see, the upper bands of the colonies 4, 5, and 6 match the upper band in the pSB2K3 size control, meaning the plasmid switch to pSB2K3 seems to have been successful. Also, the bottom bands of the colonies 4, 5, and 6 match the one in the <i>Pqrr4</i>+I13500 in pSB1AC3 lane, meaning that the construction seems to have worked. These two lower bands might look as if they do not match; however, if you consider the slanting of the ladders at right compared to the left, it seems that the two match. | As one can see, the upper bands of the colonies 4, 5, and 6 match the upper band in the pSB2K3 size control, meaning the plasmid switch to pSB2K3 seems to have been successful. Also, the bottom bands of the colonies 4, 5, and 6 match the one in the <i>Pqrr4</i>+I13500 in pSB1AC3 lane, meaning that the construction seems to have worked. These two lower bands might look as if they do not match; however, if you consider the slanting of the ladders at right compared to the left, it seems that the two match. | ||
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Revision as of 07:59, 8 August 2009
UNIVERSITY OF CALGARY
