Team:Aberdeen Scotland/notebook/lacilatch

From 2009.igem.org

(Difference between revisions)
(Week 2: BioBrick Rescue 15/06/09 - 19/06/09)
(Week 2: BioBrick Rescue 15/06/09 - 19/06/09)
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===Day 1 Monday (15/06/09)===
===Day 1 Monday (15/06/09)===
-
1. Prepared LB medium for Lab
+
*Prepared LB medium for Lab
-
2. Planning of LacI-Latch
+
*Planning of LacI-Latch
-
3. Inoculate J23100, K145001, B0034  
+
*Inoculated J23100, K145001, B0034 for Miniprep the following day
===Day 2 Tuesday (16/06/09)===
===Day 2 Tuesday (16/06/09)===
-
1. Miniprep of J23100, K145001, B0034
+
*Miniprep of J23100, K145001, B0034
-
2. Restriction Digest  
+
*Restriction Digest  
===Day 3 Wednesday (17/06/09)===
===Day 3 Wednesday (17/06/09)===
-
1. Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,   
+
*Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,   
===Day 4 Thursday (18/06/09)===
===Day 4 Thursday (18/06/09)===
-
1. Gel Electrophoresis of K112808, K112022, I732100
+
*Gel Electrophoresis of K112808, K112022, I732100
===Day 5 Friday (19/06/09)===
===Day 5 Friday (19/06/09)===
-
1. Restriction Digest of E0840
+
*Restriction Digest of E0840
-
2. Gel Electrophoresis of E0840
+
*Gel Electrophoresis of E0840
==Week 3 22/06/09 - 26/06/09==
==Week 3 22/06/09 - 26/06/09==

Revision as of 09:56, 10 August 2009

University of Aberdeen iGEM 2009


Contents

LacI-Latch Notebook

Week 1: Research and Planning 08/06/09 - 12/06/09

Day 1 Monday (08/06/09)

  • Researching the Registry for Biobricks
    • Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.


Day 2 Tuesday (09/06/09)

  • Researching the Registry for Plasmids.
    • Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.


Day 3 Wednesday (10/06/09)

  • Literature search for Parameters of T7 promoter and the Holin system.
    • Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
    • Identified the novagen plasmids pLysS and pLysE as possible solutions.


  • Researching the Registry for LacI Repressor and T7 Polymerase
    • Identified K145014, K145001, I732100 for further discusion


Day 4 Thursday (11/06/09)

  • Parameter search for the Modeling effort.


Day 5 Friday (12/06/09)

  • End of week meeting


  • Discussion and planning of following week


Week 2: BioBrick Rescue 15/06/09 - 19/06/09

Day 1 Monday (15/06/09)

  • Prepared LB medium for Lab
  • Planning of LacI-Latch
  • Inoculated J23100, K145001, B0034 for Miniprep the following day

Day 2 Tuesday (16/06/09)

  • Miniprep of J23100, K145001, B0034
  • Restriction Digest

Day 3 Wednesday (17/06/09)

  • Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,

Day 4 Thursday (18/06/09)

  • Gel Electrophoresis of K112808, K112022, I732100


Day 5 Friday (19/06/09)

  • Restriction Digest of E0840
  • Gel Electrophoresis of E0840

Week 3 22/06/09 - 26/06/09

Week 4 29/06/09 - 03/07/09

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09