Team:Aberdeen Scotland/notebook/lacilatch
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*Researching the Registry for Biobricks | *Researching the Registry for Biobricks | ||
**Identified K182022, K112808, R0062, I712074, I14015 for further disccusion. | **Identified K182022, K112808, R0062, I712074, I14015 for further disccusion. | ||
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===Day 2 Tuesday (09/06/09)=== | ===Day 2 Tuesday (09/06/09)=== | ||
Revision as of 10:31, 10 August 2009
University of Aberdeen - Pico Plumber
iGEM 2009
LacI-Latch Notebook
Week 1: Research and Planning 08/06/09 - 12/06/09
Day 1 Monday (08/06/09)
- Researching the Registry for Biobricks
- Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.
Day 2 Tuesday (09/06/09)
- Researching the Registry for Plasmids.
- Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.
Day 3 Wednesday (10/06/09)
- Literature search for Parameters of T7 promoter and the Holin system.
- Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
- Identified the novagen plasmids pLysS and pLysE as possible solutions.
- Researching the Registry for LacI Repressor and T7 Polymerase
- Identified K145014, K145001, I732100 for further discusion
Day 4 Thursday (11/06/09)
- Parameter search for the Modeling effort.
Day 5 Friday (12/06/09)
- End of week meeting
- Discussion and planning of following week
Week 2: BioBrick Rescue 15/06/09 - 19/06/09
Day 1 Monday (15/06/09)
- Prepared LB medium for Lab
- Planning of LacI-Latch
- Inoculated J23100, K145001, B0034 for Miniprep the following day
Day 2 Tuesday (16/06/09)
- Miniprep of J23100, K145001, B0034
- Restriction Digest
Day 3 Wednesday (17/06/09)
- Lambda Digest with HindIII for use as a Ladder
- Gel Electrophoresis of B0034, K145001, J23100, I732100.
Day 4 Thursday (18/06/09)
- Gel Electrophoresis of C0051, R0062, C0040, J37033, K145001, I732100.
- Innoculation of C0051, R0062, J37033, I732100 or miniprep.
Day 5 Friday (19/06/09)
- Miniprep of C0051, R0062, J37033, I732100.
- End of week meeting
Week 3: Edinburgh iGEM meeting and BioBrick Rescuing 22/06/09 - 26/06/09
Day 1 Monday (22/06/09)
- Rescued BBa_1732820, S03518, B0030
Day 2 Tuesday (23/06/09)
Risat:
- Research for Plasmids and J series Promoters
- Rescued and Transformed Psb4k4 and Psb3K5.
- Restriction Digest of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,
Daniel:
- Edinburgh Igem Meeting
Day 3 Wednesday (24/06/09)
Risat:
- Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,
Daniel:
- Edinburgh Igem Meeting
Day 4 Thursday (25/06/09)
Risat:
- Gel Electrophoresis of K112808, K112022, I732100
Daniel:
- Edinburgh Igem Meeting
Day 5 Friday (26/06/09)
- Restriction Digest of E0840
- Gel Electrophoresis of E0840
- End of week meeting
Week 4: 29/06/09 - 03/07/09
Day 1 Monday
- Run Gel of E0840 ( Testing of digests)
- Innoculate for miniprep.
- I732820 (LacI,) R0051 (CI operator), S03518 ( TetR), K112808 ( Holin), PSB3k3, PSB3T5
- Rescue I13600 and I13601
- Required for testing of constructs
Day 2 Tuesday
- Miniprep
- I732820 (LacI) R0051 (CI operator) S03518 ( TetR) K112808 ( Holin) PSB3k3, PSB3T5
- Planning for Restriction digest
- R0051, S03518, R0040, PSB3k3, I732820.
- Restriction Digest
- R0051, S03518, R0040, PSB3k3, I732820.
- Gel Electrophoresis
- R0051, S03518, R0040, PSB3k3, I732820.
- Transformation (using XL1-Blue)
- I13600, I13601
- Result : Failed (no growth)
- I13600, I13601
- Innoculation for Miniprep
- PSB4K5, PSB3K3
Day 3 Wednesday
- Miniprep
- PSB4K5, PSB3K3
- Restriction Digest
- PSB4k5, PSB3K3, S03518, R0040
- Gel electrophoresis
- PSB4K5, PSB3K3, S03518, R0040
- Innoculate for Miniprep
- PSB1AT3, PSB1AC3
Day 4 Thursday
- Transformation (using Xl1-Blue)
- I13600 and I13601
- Result : Worked
- I13600 and I13601
- Gel Electrophoresis
- R0040
- Incoculate for Miniprep
- PSB3AT3, PSB3AC3
Day 5 Friday
- Miniprep
- PSB3AT3, PSB3AC3
- End of week meeting
Week 5 05/07/09 - 10/07/09
Week 6 13/07/09 - 16/07/09
Week 7 20/07/09 - 24/07/09
Week 8 27/07/09 - 31/07/09
Week 9 03/08/09 - 07/08/09
Week 10 10/08/09 - 14/08/09
Week 11 17/08/09 - 21/08/09
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