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Virginia Commonwealth/9 July 2009
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==Thursday 9 July 2009== | ==Thursday 9 July 2009== | ||
===Results=== | ===Results=== | ||
''Afton and Maria'' | ''Afton and Maria'' | ||
*Electrotransformation was successful | *Electrotransformation was successful | ||
| - | + | ||
| - | + | {| cellpadding="5" border="1" padding="5" | |
| - | + | |+ | |
| - | + | |- | |
| - | + | ! Name !! Plate !! Growth Observation | |
| + | |- | ||
| + | | Control (+) NEB10β || LB || lawn | ||
| + | |- | ||
| + | | Control (+) NEB10β shocked || LB || lawn | ||
| + | |- | ||
| + | | Control (-) NEB10β || Amp || no growth | ||
| + | |- | ||
| + | | J23106 (promoter) || Amp || hundreds of colonies | ||
| + | |- | ||
| + | | J06702 (RET) || Amp || ~ one hundred colonies | ||
| + | |} | ||
[[User:Trentay|Trentay]] 14:06, 9 July 2009 (UTC) | [[User:Trentay|Trentay]] 14:06, 9 July 2009 (UTC) | ||
| - | |||
===Tasks=== | ===Tasks=== | ||
| Line 20: | Line 68: | ||
''Craig and Clay'' | ''Craig and Clay'' | ||
| - | * The three parts for limonene synthesis ( | + | * The three parts for limonene synthesis (BBa_I742111, BBa_K118024, and BBa_K118025) will be electrotransformed today. They will also be plated and grown overnight on ampicillin-selective media. |
* Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts. | * Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts. | ||
---- | ---- | ||
| Line 28: | Line 76: | ||
* Put 5 colonies of J06702 in 5mL of LB (one test tube) | * Put 5 colonies of J06702 in 5mL of LB (one test tube) | ||
[[User:Trentay|Trentay]] 21:13, 9 July 2009 (UTC) | [[User:Trentay|Trentay]] 21:13, 9 July 2009 (UTC) | ||
| + | |||
| + | ''Craig and Clay'' | ||
| + | * The following parts for limonene synthesis were electrotransformed into the NEB 10 beta strain of E coli: BBa_I742111, BBa_K118024, and BBa_K118025. All DNA was obtained from the distribution plates. These electrotransformed parts were then plated on ampicillin-selective plates and left to grow overnight. | ||
Latest revision as of 16:18, 10 August 2009
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Contents |
Thursday 9 July 2009
Results
Afton and Maria
- Electrotransformation was successful
| Name | Plate | Growth Observation |
|---|---|---|
| Control (+) NEB10β | LB | lawn |
| Control (+) NEB10β shocked | LB | lawn |
| Control (-) NEB10β | Amp | no growth |
| J23106 (promoter) | Amp | hundreds of colonies |
| J06702 (RET) | Amp | ~ one hundred colonies |
Trentay 14:06, 9 July 2009 (UTC)
Tasks
Afton and Maria
- Pick cells for overnight culture
Trentay 19:45, 9 July 2009 (UTC)
Kevin and Adam
- Proceed with analytical work and ordering DNA.
Craig and Clay
- The three parts for limonene synthesis (BBa_I742111, BBa_K118024, and BBa_K118025) will be electrotransformed today. They will also be plated and grown overnight on ampicillin-selective media.
- Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts.
Wetlab
Afton and Maria
- Put 5 colonies of J23106 in 5mL of LB (two test tube)
- Put 5 colonies of J06702 in 5mL of LB (one test tube)
Trentay 21:13, 9 July 2009 (UTC)
Craig and Clay
- The following parts for limonene synthesis were electrotransformed into the NEB 10 beta strain of E coli: BBa_I742111, BBa_K118024, and BBa_K118025. All DNA was obtained from the distribution plates. These electrotransformed parts were then plated on ampicillin-selective plates and left to grow overnight.
