Virginia Commonwealth/16 July 2009

From 2009.igem.org

(Difference between revisions)

Latest revision as of 18:17, 13 August 2009

May
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				[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14]	[http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23]	[http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]

June
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[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]

July
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		[http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30]	[http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]

August
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					[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4]	[http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11]	[http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15]	[http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18]	[http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]

September
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	[http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2]	[http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3]	[http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9]	[http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10]	[http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25]	[http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]

October
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			[http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1]	[http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5]	[http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6]	[http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7]	[http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8]	[http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12]	[http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13]	[http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14]	[http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15]	[http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16]	[http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17]	[http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19]	[http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20]	[http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21]	[http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22]	[http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23]	[http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24]	[http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26]	[http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27]	[http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28]	[http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29]	[http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30]	[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Thursday 16 July 2009

Results

Maria and Afton

Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation


Promoter	Reporter	Plate	Growth Observation
J23101	E0240	Cm	no growth
J23101	J06702	Cm	21 colonies
J23102	E0240	Cm	3 colonies
J23102	J06702	Cm	8 colonies
J23103	E0240	Cm	2 colonies
J23103	J06702	Cm	23 colonies
J23104	E0240	Cm	9 colonies
J23104	J06702	Cm	12 colonies
J23105	E0240	Cm	no growth
J23105	J06702	Cm	14 colonies
J23107	E0240	Cm	3 colonies
J23107	J06702	Cm	3 colonies

No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
- Vial may have thawed out too much after cells were taken out for the electrocompetency procedure

Spectrophotometry measurements after Miniprep


Part No.	A260 (nm)	A280 (nm)	A260/A280	Vol. (µL)	Conc. (µg/mL)	Amt (µg)
J23110	0.050	0.038	1.32	48	75	3.60
J23113	0.041	0.030	1.37	44	21	0.92
J23116	0.029	0.023	1.26	44	43	1.91
R0011	0.022	0.020	1.10	41	33	1.35
R0040	0.024	0.021	1.14	42	36	1.51
E0240	0.056	0.036	1.56	109	84	9.16
pSB4C5	0.116	0.066	1.76	174	22	3.83
J06702	0.042	0.024	1.75	63	130	8.19

Gel Electrophoresis Results

100bp Ladder, Digestions on the Gel: J23110, J23113, J23116, R0011, R0040, E0240

Trentay 14:31, 16 July 2009 (UTC)

Craig and Clay

No growth was seen on either the kanamycin or chloramphenicol plates.
The sequence for the LIM1 gene was verified to be optimized for E. coli.

Tasks

Maria and Afton

Spectrophotometry
Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
Run an electrophoresis test on promoters and reporter Eo240
Pick colonies of promoters from transformation 100-107 (excluding 106)
Transformation of NEB 10 beta to make sure new stock is electrocompetent

Trentay 20:41, 16 July 2009 (UTC)

Craig and Clay

The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.

Wetlab

Maria and Afton

Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
Run an electrophoresis test on promoters and reporter Eo240

Trentay 20:41, 16 July 2009 (UTC)

@@ Line 1: / Line 1: @@
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+<html>
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+table.month .dow td     { color:#000000; text-align:center; font-size:100%; }
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+table.month td {
+    border: none;
+    margin: 0;
+    padding: 0pt 0.5pt;
+    font-weight: bold;
+    font-size: 10pt;
+    text-align: right;
+    background-color: white;
+    }
+#bodyContent table.month a { background:none; padding:0 }
+.day-active { color:#cd0000 }
+.day-empty  { color:#000000 }
+</style>
+</html>
+{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:center" width="250px"
+</table>
+{| align="center"
+|-valign="top"
+|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=05}}
+|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=06}}
+|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=07}}
+|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=08}}
+|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=09}}
+|align="center" width="150pt"|{{#calendar: title=Virginia_Commonwealth |year=2009 | month=10}}
+|}
 ==Thursday 16 July 2009==
 ===Results===
+''Maria and Afton''
 * Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
-{| cellpadding="10" align="center" border="0.9" padding="5"
+{| cellpadding="5" border="1" padding="5"
 |+
 |-
-! Promoter!! Reporter !! Resistance/Plate !! Growth Observation !!
+! Promoter!! Reporter !! Plate !! Growth Observation
 |-
 ! J23101
-| E0240 || Cm || no growth ||
+| E0240 || Cm || no growth
 |-
 ! J23101
-| J06702 || Cm || 21 colonies ||
+| J06702 || Cm || 21 colonies
 |-
 ! J23102
-| E0240 || Cm || 3 colonies ||
+| E0240 || Cm || 3 colonies
 |-
 ! J23102
-| J06702 || Cm || 8 colonies ||
+| J06702 || Cm || 8 colonies
 |-
 ! J23103
-| E0240 || Cm || 2 colonies ||
+| E0240 || Cm || 2 colonies
 |-
 ! J23103
-| J06702 || Cm || 23 colonies ||
+| J06702 || Cm || 23 colonies
 |-
 ! J23104
-| E0240 || Cm || 9 colonies ||
+| E0240 || Cm || 9 colonies
 |-
 ! J23104
-| J06702 || Cm || 12 colonies ||
+| J06702 || Cm || 12 colonies
 |-
 ! J23105
-| E0240 || Cm || no growth ||
+| E0240 || Cm || no growth
 |-
 ! J23105
-| J06702 || Cm || 14 colonies ||
+| J06702 || Cm || 14 colonies
 |-
 ! J23107
-| E0240 || Cm || 3 colonies ||
+| E0240 || Cm || 3 colonies
 |-
 ! J23107
-| J06702 || Cm || 3 colonies ||
+| J06702 || Cm || 3 colonies
 |}
 * No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
 **Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
+* Spectrophotometry measurements after Miniprep
+{| cellpadding="5" border="1" padding="5"
+|+
+|-
+! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !!  Vol. (µL) !! Conc. (µg/mL) !! Amt (µg)
+|-
+| J23110 || 0.050 || 0.038 || 1.32 || 48 || 75 || 3.60
+|-
+| J23113 || 0.041 || 0.030 || 1.37 || 44 || 21 || 0.92
+|-
+| J23116 || 0.029 || 0.023 || 1.26 || 44 || 43 || 1.91
+|-
+| R0011 || 0.022 || 0.020 || 1.10 || 41 || 33 || 1.35
+|-
+| R0040 || 0.024 || 0.021 || 1.14 || 42 || 36 || 1.51
+|-
+| E0240 || 0.056 || 0.036 || 1.56 || 109 || 84 || 9.16
+|-
+| pSB4C5 || 0.116 || 0.066 || 1.76 || 174 || 22 || 3.83
+|-
+| J06702 || 0.042 || 0.024 || 1.75 || 63 || 130 || 8.19
+|}
+* Gel Electrophoresis Results
+[[Image:2009-07-16 digest07 110,113,116,0011,0040,240.jpg|350px|thumb|none| 100bp Ladder, Digestions on the Gel: J23110, J23113, J23116, R0011, R0040, E0240]]
 [[User:Trentay|Trentay]] 14:31, 16 July 2009 (UTC)
---Craig and Clay--
+''Craig and Clay''
 * No growth was seen on either the kanamycin or chloramphenicol plates.
 * The sequence for the LIM1 gene was verified to be optimized for E. coli.
@@ Line 55: / Line 121: @@
 ===Tasks===
 ''Maria and Afton''
+* Spectrophotometry
 * Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
 * Ligate promoters with backbone pSB4C5 and reporters E0240, J06702

Virginia Commonwealth/16 July 2009

From 2009.igem.org

Latest revision as of 18:17, 13 August 2009

Contents

Thursday 16 July 2009

Results

Tasks

Wetlab