Virginia Commonwealth/16 July 2009

From 2009.igem.org

(Difference between revisions)
(→Results)
 
(3 intermediate revisions not shown)
Line 39: Line 39:
==Thursday 16 July 2009==
==Thursday 16 July 2009==
===Results===
===Results===
 +
''Maria and Afton''
* Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
* Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
-
{| cellpadding="10" align="center" border="0.9" padding="5"
+
{| cellpadding="5" border="1" padding="5"
|+  
|+  
|-
|-
-
! Promoter!! Reporter !! Resistance/Plate !! Growth Observation !!
+
! Promoter!! Reporter !! Plate !! Growth Observation  
|-
|-
! J23101  
! J23101  
-
| E0240 || Cm || no growth ||
+
| E0240 || Cm || no growth  
|-
|-
! J23101  
! J23101  
-
| J06702 || Cm || 21 colonies ||
+
| J06702 || Cm || 21 colonies  
|-
|-
! J23102  
! J23102  
-
| E0240 || Cm || 3 colonies ||
+
| E0240 || Cm || 3 colonies  
|-
|-
! J23102  
! J23102  
-
| J06702 || Cm || 8 colonies ||
+
| J06702 || Cm || 8 colonies  
|-
|-
! J23103  
! J23103  
-
| E0240 || Cm || 2 colonies ||
+
| E0240 || Cm || 2 colonies  
|-
|-
! J23103
! J23103
-
| J06702 || Cm || 23 colonies ||
+
| J06702 || Cm || 23 colonies  
|-
|-
! J23104  
! J23104  
-
| E0240 || Cm || 9 colonies ||
+
| E0240 || Cm || 9 colonies  
|-
|-
! J23104  
! J23104  
-
| J06702 || Cm || 12 colonies ||
+
| J06702 || Cm || 12 colonies  
|-
|-
! J23105  
! J23105  
-
| E0240 || Cm || no growth ||
+
| E0240 || Cm || no growth  
|-
|-
! J23105  
! J23105  
-
| J06702 || Cm || 14 colonies ||
+
| J06702 || Cm || 14 colonies  
|-
|-
! J23107  
! J23107  
-
| E0240 || Cm || 3 colonies ||
+
| E0240 || Cm || 3 colonies  
|-
|-
! J23107
! J23107
-
| J06702 || Cm || 3 colonies ||
+
| J06702 || Cm || 3 colonies  
|}
|}
* No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
* No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
**Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
**Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
 +
 +
* Spectrophotometry measurements after Miniprep
 +
{| cellpadding="5" border="1" padding="5"
 +
|+
 +
|-
 +
! Part No.!! A260 (nm) !! A280 (nm) !! A260/A280 !!  Vol. (µL) !! Conc. (µg/mL) !! Amt (µg)
 +
|-
 +
| J23110 || 0.050 || 0.038 || 1.32 || 48 || 75 || 3.60
 +
|-
 +
| J23113 || 0.041 || 0.030 || 1.37 || 44 || 21 || 0.92
 +
|-
 +
| J23116 || 0.029 || 0.023 || 1.26 || 44 || 43 || 1.91
 +
|-
 +
| R0011 || 0.022 || 0.020 || 1.10 || 41 || 33 || 1.35
 +
|-
 +
| R0040 || 0.024 || 0.021 || 1.14 || 42 || 36 || 1.51
 +
|-
 +
| E0240 || 0.056 || 0.036 || 1.56 || 109 || 84 || 9.16
 +
|-
 +
| pSB4C5 || 0.116 || 0.066 || 1.76 || 174 || 22 || 3.83
 +
|-
 +
| J06702 || 0.042 || 0.024 || 1.75 || 63 || 130 || 8.19
 +
|}
 +
 +
* Gel Electrophoresis Results
 +
[[Image:2009-07-16 digest07 110,113,116,0011,0040,240.jpg|350px|thumb|none| 100bp Ladder, Digestions on the Gel: J23110, J23113, J23116, R0011, R0040, E0240]]
 +
[[User:Trentay|Trentay]] 14:31, 16 July 2009 (UTC)
[[User:Trentay|Trentay]] 14:31, 16 July 2009 (UTC)
Line 93: Line 121:
===Tasks===
===Tasks===
''Maria and Afton''
''Maria and Afton''
 +
* Spectrophotometry
* Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
* Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
* Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
* Ligate promoters with backbone pSB4C5 and reporters E0240, J06702

Latest revision as of 18:17, 13 August 2009

May
MTWTFSS
        [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_May_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_May_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_May_2009&action=edit 3]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_May_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_May_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_May_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/7_May_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_May_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_May_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_May_2009&action=edit 10]
[http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_May_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_May_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_May_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/14_May_2009&action=edit 14] [http://2009.igem.org/Virginia_Commonwealth/15_May_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_May_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_May_2009 17]
[http://2009.igem.org/Virginia_Commonwealth/18_May_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_May_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_May_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_May_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_May_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_May_2009&action=edit 23] [http://2009.igem.org/Virginia_Commonwealth/24_May_2009 24]
[http://2009.igem.org/Virginia_Commonwealth/25_May_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_May_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_May_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_May_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_May_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_May_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_May_2009&action=edit 31]
June
MTWTFSS
[http://2009.igem.org/Virginia_Commonwealth/1_June_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_June_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_June_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_June_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_June_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_June_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_June_2009 7]
[http://2009.igem.org/Virginia_Commonwealth/8_June_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_June_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_June_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_June_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_June_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_June_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_June_2009 14]
[http://2009.igem.org/Virginia_Commonwealth/15_June_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_June_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_June_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_June_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_June_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_June_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_June_2009 21]
[http://2009.igem.org/Virginia_Commonwealth/22_June_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_June_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_June_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_June_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_June_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_June_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_June_2009 28]
[http://2009.igem.org/Virginia_Commonwealth/29_June_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_June_2009 30]
July
MTWTFSS
    [http://2009.igem.org/Virginia_Commonwealth/1_July_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_July_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_July_2009&action=edit 5]
[http://2009.igem.org/Virginia_Commonwealth/6_July_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_July_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_July_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_July_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_July_2009&action=edit 12]
[http://2009.igem.org/Virginia_Commonwealth/13_July_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_July_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_July_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_July_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_July_2009&action=edit 19]
[http://2009.igem.org/Virginia_Commonwealth/20_July_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_July_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_July_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_July_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/26_July_2009&action=edit 26]
[http://2009.igem.org/Virginia_Commonwealth/27_July_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_July_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_July_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_July_2009 30] [http://2009.igem.org/Virginia_Commonwealth/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/2_August_2009&action=edit 2]
[http://2009.igem.org/Virginia_Commonwealth/3_August_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_August_2009 4] [http://2009.igem.org/Virginia_Commonwealth/5_August_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_August_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_August_2009 7] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/8_August_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/9_August_2009&action=edit 9]
[http://2009.igem.org/Virginia_Commonwealth/10_August_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_August_2009 11] [http://2009.igem.org/Virginia_Commonwealth/12_August_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_August_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/15_August_2009&action=edit 15] [http://2009.igem.org/Virginia_Commonwealth/16_August_2009 16]
[http://2009.igem.org/Virginia_Commonwealth/17_August_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_August_2009 18] [http://2009.igem.org/Virginia_Commonwealth/19_August_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_August_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_August_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_August_2009 22] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/23_August_2009&action=edit 23]
[http://2009.igem.org/Virginia_Commonwealth/24_August_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_August_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_August_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_August_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_August_2009 28] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/29_August_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/30_August_2009&action=edit 30]
[http://2009.igem.org/Virginia_Commonwealth/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Virginia_Commonwealth/1_September_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_September_2009 2] [http://2009.igem.org/Virginia_Commonwealth/3_September_2009 3] [http://2009.igem.org/Virginia_Commonwealth/4_September_2009 4] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/5_September_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/6_September_2009&action=edit 6]
[http://2009.igem.org/Virginia_Commonwealth/7_September_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_September_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_September_2009 9] [http://2009.igem.org/Virginia_Commonwealth/10_September_2009 10] [http://2009.igem.org/Virginia_Commonwealth/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/13_September_2009&action=edit 13]
[http://2009.igem.org/Virginia_Commonwealth/14_September_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_September_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_September_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_September_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_September_2009 18] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/19_September_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/20_September_2009&action=edit 20]
[http://2009.igem.org/Virginia_Commonwealth/21_September_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_September_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_September_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_September_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_September_2009 25] [http://2009.igem.org/Virginia_Commonwealth/26_September_2009 26] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/27_September_2009&action=edit 27]
[http://2009.igem.org/Virginia_Commonwealth/28_September_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_September_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/Virginia_Commonwealth/1_October_2009 1] [http://2009.igem.org/Virginia_Commonwealth/2_October_2009 2] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/3_October_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/4_October_2009&action=edit 4]
[http://2009.igem.org/Virginia_Commonwealth/5_October_2009 5] [http://2009.igem.org/Virginia_Commonwealth/6_October_2009 6] [http://2009.igem.org/Virginia_Commonwealth/7_October_2009 7] [http://2009.igem.org/Virginia_Commonwealth/8_October_2009 8] [http://2009.igem.org/Virginia_Commonwealth/9_October_2009 9] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/10_October_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/11_October_2009&action=edit 11]
[http://2009.igem.org/Virginia_Commonwealth/12_October_2009 12] [http://2009.igem.org/Virginia_Commonwealth/13_October_2009 13] [http://2009.igem.org/Virginia_Commonwealth/14_October_2009 14] [http://2009.igem.org/Virginia_Commonwealth/15_October_2009 15] [http://2009.igem.org/Virginia_Commonwealth/16_October_2009 16] [http://2009.igem.org/Virginia_Commonwealth/17_October_2009 17] [http://2009.igem.org/Virginia_Commonwealth/18_October_2009 18]
[http://2009.igem.org/Virginia_Commonwealth/19_October_2009 19] [http://2009.igem.org/Virginia_Commonwealth/20_October_2009 20] [http://2009.igem.org/Virginia_Commonwealth/21_October_2009 21] [http://2009.igem.org/Virginia_Commonwealth/22_October_2009 22] [http://2009.igem.org/Virginia_Commonwealth/23_October_2009 23] [http://2009.igem.org/Virginia_Commonwealth/24_October_2009 24] [http://2009.igem.org/Virginia_Commonwealth/25_October_2009 25]
[http://2009.igem.org/Virginia_Commonwealth/26_October_2009 26] [http://2009.igem.org/Virginia_Commonwealth/27_October_2009 27] [http://2009.igem.org/Virginia_Commonwealth/28_October_2009 28] [http://2009.igem.org/Virginia_Commonwealth/29_October_2009 29] [http://2009.igem.org/Virginia_Commonwealth/30_October_2009 30] [http://2009.igem.org/wiki/index.php?title=Virginia_Commonwealth/31_October_2009&action=edit 31]

Contents

Thursday 16 July 2009

Results

Maria and Afton

  • Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation
Promoter Reporter Plate Growth Observation
J23101 E0240 Cm no growth
J23101 J06702 Cm 21 colonies
J23102 E0240 Cm 3 colonies
J23102 J06702 Cm 8 colonies
J23103 E0240 Cm 2 colonies
J23103 J06702 Cm 23 colonies
J23104 E0240 Cm 9 colonies
J23104 J06702 Cm 12 colonies
J23105 E0240 Cm no growth
J23105 J06702 Cm 14 colonies
J23107 E0240 Cm 3 colonies
J23107 J06702 Cm 3 colonies
  • No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
    • Vial may have thawed out too much after cells were taken out for the electrocompetency procedure
  • Spectrophotometry measurements after Miniprep
Part No. A260 (nm) A280 (nm) A260/A280 Vol. (µL) Conc. (µg/mL) Amt (µg)
J23110 0.050 0.038 1.32 48 75 3.60
J23113 0.041 0.030 1.37 44 21 0.92
J23116 0.029 0.023 1.26 44 43 1.91
R0011 0.022 0.020 1.10 41 33 1.35
R0040 0.024 0.021 1.14 42 36 1.51
E0240 0.056 0.036 1.56 109 84 9.16
pSB4C5 0.116 0.066 1.76 174 22 3.83
J06702 0.042 0.024 1.75 63 130 8.19
  • Gel Electrophoresis Results
100bp Ladder, Digestions on the Gel: J23110, J23113, J23116, R0011, R0040, E0240

Trentay 14:31, 16 July 2009 (UTC)

Craig and Clay

  • No growth was seen on either the kanamycin or chloramphenicol plates.
  • The sequence for the LIM1 gene was verified to be optimized for E. coli.

Tasks

Maria and Afton

  • Spectrophotometry
  • Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
  • Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
  • Run an electrophoresis test on promoters and reporter Eo240
  • Pick colonies of promoters from transformation 100-107 (excluding 106)
  • Transformation of NEB 10 beta to make sure new stock is electrocompetent

Trentay 20:41, 16 July 2009 (UTC)

Craig and Clay

  • The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.

Wetlab

Maria and Afton

  • Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
  • Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
  • Run an electrophoresis test on promoters and reporter Eo240

Trentay 20:41, 16 July 2009 (UTC)

Retrieved from "http://2009.igem.org/Virginia_Commonwealth/16_July_2009"