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Uppsala-Sweden/14 August 2009
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Karl.brune (Talk | contribs) (New page: {{Uppsala-Sweden Template}} ==Transformation of Adh2 z, evaluation of competence== ''kontinued since yesterday'' So, it's official, our competent cells are really quite ''in''competent! ...) |
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==Transformation of Adh2 z, evaluation of competence== | ==Transformation of Adh2 z, evaluation of competence== | ||
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So, it's official, our competent cells are really quite ''in''competent! Tested against the new bath of competent DH5alfa which got 220 transformations (100*10^6 / µg pUC 19)our perfored extremly bad at around 20 transformations. | So, it's official, our competent cells are really quite ''in''competent! Tested against the new bath of competent DH5alfa which got 220 transformations (100*10^6 / µg pUC 19)our perfored extremly bad at around 20 transformations. | ||
Revision as of 14:29, 14 August 2009
Transformation of Adh2 z, evaluation of competence
continued since yesterday
So, it's official, our competent cells are really quite incompetent! Tested against the new bath of competent DH5alfa which got 220 transformations (100*10^6 / µg pUC 19)our perfored extremly bad at around 20 transformations.
We will make new CCMB80 (unauotclaved this time) and also consider to switch cells to TOP10 instead.
--Karl.brune 14:28, 14 August 2009 (UTC)
Plasmid prep contiued
Yesterday we performed a plasmid prep on all transformed genes. Today I did the same for the B0034 RBS. This with a small screw up as I accedentilly eluted with 1 M Tris-HCl as opposed to 5 mM! I precipitated the DNA wizh ethanol and resuspen in 5 mM Tris-HCl.
Left to do: Measure DNA koncentration for all the plasmid preps.
--Karl.brune 14:28, 14 August 2009 (UTC)
