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Team:Calgary/14 August 2009
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| - | + | Verifying the fluorescence of the cells with reporter and mutant circuit | |
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| - | + | The cells with reporter and mutant circuits were viewed under UV in order to see if the mutants and the reporter circuits are properly functional. However, no colonies were found to be glowing. Since the level of GFP production is low due to the inducible low copy plasmid that it is in, we may have to grow an overnight culture and measure the fluorescence of the culture with the plate reader. If the fluorescence is found to be too low to decide whether or not the circuits are properly functioning, we may have to induce the cells with IPTG to induce the duplication of the reporter circuit. This would hopefully increase the production levels of GFP because more Pqrr4 promoters would be available for the mutant proteins to attach to. | |
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Revision as of 23:45, 14 August 2009
UNIVERSITY OF CALGARY
