Team:Calgary/10 July 2009

• Performed a colony PCR of J13002-LuxOD47E-B0015 to see if the B0015 terminator was seuccessfully cloned in, using BBK-CP forward and reverse primers and p Taq. Cycling conditions were the same as used for the last Colony PCR (see July 9th 2009 for details). PCR products were visualized on a 1% agarose gel and run with Generuler 1 kb+ DNA ladder along with J13002-LuxOD47E and LuxOD47E as size controls. See photo of gel below.

• Analysis: Lanes 1-4 are J13002-LuxOD47E-B0015 trial 1 (treating B0015 as the Insert). Lanes 5-8 are trial 2 (treating J13002-LuxOD47E as the Insert). Lane 9 is J13002-LuxOD47E (a size control), Lane 10 is LuxOD47E BBK (another size control) and Lane 11 is a negative control.

• This gel seems ot be kind of slanted, which isn't very good for size comparison. It looks like some of the first lanes of Trial 1 may have worked (contain the B0015 terminator) as they appear slighlty higher than other lanes, however we will run a new gel with the PCR product to be sure.

Yesterday, time was spent on building a robot (Biobert) that will act as a guide to our lab and also the rest of the island. It had to be built from scratch because the first one wasn't very good and creeped people out because it transformed from a pipette and had an empty soulless expression. Once it was built, Mandy added the script so it could talk to people (including several secret phrases!). I designed it to move its arms and mouth when touched and optimized to use the least prims possible in order to make sure we have enough left for other things on the island. Today, a second robot was also made in the same fashion (Synthia) because Biobert was lonely and she needed a friend.