From 2009.igem.org
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Revision as of 15:47, 21 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Stochastic Models of Gene Expression
Stochastic mechanisms in gene expression
A stochastic model of Escherichia coli AI-2 quorum signal circuit reveals alternative synthesis pathways --- has stochastic rate constants
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
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MANDY
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PATRICK
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PRIMA
Continuation of Marketing
I shadowed Carol while she did restriction digest. She was doing construction. Her vector was apsB1AK3. However, since I didn't go into the lab very often, I wasn't sure what she was digesting. I just watched the processes and she just explained the restriction sites.
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STEFAN
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VICKI
Colony PCR of J13002+LuxOD47A and LuxOD47A+B0015 bacteria
Purpose: Cultures grew successfully on plates last night. This will help confirm which of those colonies took up the plasmids that we wanted to, and that the plasmids that were taken up include properly-constructed genes in the right order.
Protocol: The colony PCR was conducted in accordance with that outlined on the protocol page. Platinum Taq polymerase was used, along with BBk construction primers. 5 colonies were tested for each construct, along with one negative control (for a total of 11 tubes being amplified).
Results: The gel is included below. The negative control results are peculiar, but are not raising any concern as the contamination band is not found anywhere else on the gel. Some of the LuxOD47A stock was run to serve as a size comparison. From this, it appears that the LuxOD47A+B0015 parts were successfully constructed and transformed, along with colonies 2 and 4 of the J13002+LuxOD47A parts.
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