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Minnesota/30 June 2009
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| + | '''Patrick'''<br> | ||
| + | Here is the "leaky" graph along with the base for comparison:<br> | ||
| + | |||
| + | <center><gallery widths=300 heights=200> | ||
| + | Image:badBase.jpg|Figure 1 - Base TTN Model | ||
| + | Image:Leak.jpg|Figure 2 - Leaky Model | ||
| + | </gallery></center><br> | ||
| + | |||
| + | As can be seen the "leaky" graph indeed does show increased GFP production at low aTc concentrations.<br> | ||
| + | |||
| + | '''Ben'''<br> | ||
| + | {| class="wikitable" style="text-align:center" border = "1" | ||
| + | |- | ||
| + | ! Reaction | ||
| + | ! Forward Kinetic Constant | ||
| + | ! Reverse Kinetic Constant | ||
| + | |- | ||
| + | |RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |- | ||
| + | |RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |- | ||
| + | ||RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |} | ||
| + | [[Image:Tnnatcvariesleaky11.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> | ||
| + | These graphs show almost no change from yesterday's graph, even though the kinetic constant has been reduced by an order of magnitude. This is probably due to the fact that the constant is so low already, and so making it even lower will have little effect. | ||
Latest revision as of 16:21, 25 August 2009
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| Go to Previous Day (June 29) | Go to Next Day (July 1) |
Patrick
Here is the "leaky" graph along with the base for comparison:
 Figure 1 - Base TTN Model |
 Figure 2 - Leaky Model |
As can be seen the "leaky" graph indeed does show increased GFP production at low aTc concentrations.
Ben
| Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
|---|---|---|
| RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 | |
| RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 | |
| RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 |
These graphs show almost no change from yesterday's graph, even though the kinetic constant has been reduced by an order of magnitude. This is probably due to the fact that the constant is so low already, and so making it even lower will have little effect.
