"
Minnesota/30 June 2009
From 2009.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 5: | Line 5: | ||
|'''[[Minnesota/29 June 2009|Go to Previous Day (June 29)]]'''|| width=158|'''[[Minnesota/1 July 2009|Go to Next Day (July 1)]]''' | |'''[[Minnesota/29 June 2009|Go to Previous Day (June 29)]]'''|| width=158|'''[[Minnesota/1 July 2009|Go to Next Day (July 1)]]''' | ||
|} | |} | ||
| - | <br> | + | '''Patrick'''<br> |
| + | Here is the "leaky" graph along with the base for comparison:<br> | ||
| + | |||
| + | <center><gallery widths=300 heights=200> | ||
| + | Image:badBase.jpg|Figure 1 - Base TTN Model | ||
| + | Image:Leak.jpg|Figure 2 - Leaky Model | ||
| + | </gallery></center><br> | ||
| + | |||
| + | As can be seen the "leaky" graph indeed does show increased GFP production at low aTc concentrations.<br> | ||
| + | |||
| + | '''Ben'''<br> | ||
| + | {| class="wikitable" style="text-align:center" border = "1" | ||
| + | |- | ||
| + | ! Reaction | ||
| + | ! Forward Kinetic Constant | ||
| + | ! Reverse Kinetic Constant | ||
| + | |- | ||
| + | |RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |- | ||
| + | |RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |- | ||
| + | ||RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2||30|| | ||
| + | |} | ||
[[Image:Tnnatcvariesleaky11.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> | [[Image:Tnnatcvariesleaky11.jpg|480px]][[Image:Tnnexperiment.jpg|480px]]<br> | ||
| + | These graphs show almost no change from yesterday's graph, even though the kinetic constant has been reduced by an order of magnitude. This is probably due to the fact that the constant is so low already, and so making it even lower will have little effect. | ||
Latest revision as of 16:21, 25 August 2009
| Back to Notebook Home | |
| Go to Previous Day (June 29) | Go to Next Day (July 1) |
Patrick
Here is the "leaky" graph along with the base for comparison:
 Figure 1 - Base TTN Model |
 Figure 2 - Leaky Model |
As can be seen the "leaky" graph indeed does show increased GFP production at low aTc concentrations.
Ben
| Reaction | Forward Kinetic Constant | Reverse Kinetic Constant |
|---|---|---|
| RNAp + tetO1:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 | |
| RNAp + tetO1:aTc:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 | |
| RNAp + tetO1:aTc2:tetR2 + lacP -> RNAp:lacP + tetR2 | 30 |
These graphs show almost no change from yesterday's graph, even though the kinetic constant has been reduced by an order of magnitude. This is probably due to the fact that the constant is so low already, and so making it even lower will have little effect.
