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Template:Team:KULeuven/20 August 2009/VanillinReceptor
From 2009.igem.org
(Difference between revisions)
(New page: * Continued with ethanol precipitation, electroporation and outplating (of R mutation 202)) |
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| - | * | + | * Restriction digest of X, Y and K145201 with EcoR1 and Pst1 |
| + | * Gelelectroforesis to check fragments and gelextraction | ||
| + | * Fluid medium from outplated cells (overgrowth) | ||
| + | * TOPO-cloning (with new protocol from invitrogen) for W and Z | ||
Revision as of 12:53, 26 August 2009
- Restriction digest of X, Y and K145201 with EcoR1 and Pst1
- Gelelectroforesis to check fragments and gelextraction
- Fluid medium from outplated cells (overgrowth)
- TOPO-cloning (with new protocol from invitrogen) for W and Z
