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From 2009.igem.org
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- 19:00, 20 October 2009 (diff | hist) N Template:Team:KULeuven/9 October 2009/VanillinReceptor (New page: * Miniprepped samples of virA and virB were sended for sequencing and glycerolstocks were made) (top)
- 18:59, 20 October 2009 (diff | hist) N Template:Team:KULeuven/30 September 2009/VanillinReceptor (New page: * virA and virB were miniprepped) (top)
- 18:52, 20 October 2009 (diff | hist) Template:Team:KULeuven/24 September 2009/VanillinReceptor (top)
- 18:51, 20 October 2009 (diff | hist) N Template:Team:KULeuven/29 September 2009/VanillinReceptor (New page: * electropoation of virA was succesfull so it was ented * virB was ented from the motherplate) (top)
- 18:48, 20 October 2009 (diff | hist) N Template:Team:KULeuven/24 September 2009/VanillinReceptor (New page: * Again there was no cutting seen on the two samples of virA so we just started electroporation and we hoped with sequencing later one we could see if it was well mutagenated. * The mother...)
- 18:46, 20 October 2009 (diff | hist) N Template:Team:KULeuven/21 September 2009/VanillinReceptor (New page: * Again PstI din't cut overnight in both samples. A new restriction digest was done over one day because pstI did need maye more time to cut. * Electroporation of virB showed good results ...) (top)
- 18:43, 20 October 2009 (diff | hist) N Template:Team:KULeuven/18 September 2009/VanillinReceptor (New page: * We checked if the PstI had cut the mutagenated places by gel-electrophoresis but we forgot to add a sample as positive control so the restriction digest was done again. The result we put...) (top)
- 18:39, 20 October 2009 (diff | hist) N Template:Team:KULeuven/17 September 2009/VanillinReceptor (New page: * New PCR showed a better result for virA and geleclectrophoresis showed some result. The motherstrain was cut with DpnI to remove those residues, PCR-purification was performed and a rest...) (top)
- 18:36, 20 October 2009 (diff | hist) N Template:Team:KULeuven/16 September 2009/VanillinReceptor (New page: * Gel-electrophoresis showed bad result for the mutation of virA probably due to too long elongation time. So we performed this PCR again with a new mutation. * Ligation of virB didn't sho...) (top)
- 18:32, 20 October 2009 (diff | hist) N Template:Team:KULeuven/15 September 2009/VanillinReceptor (New page: * Because virA was now properly inserted in the pucvector we could now start mutagenesis on restriction sites 187 and 720. We did this with PCR. We used a protocol for site-directed-mutage...) (top)
- 18:22, 20 October 2009 (diff | hist) Template:Team:KULeuven/14 September 2009/VanillinReceptor (top)
- 13:00, 17 October 2009 (diff | hist) N Template:Team:KULeuven/14 September 2009/VanillinReceptor (New page: * miniprep of virA in the uc vector, fragment was digested a the gelelectrophoresis showed a good result * new restriction digest for virB was done)
- 12:57, 17 October 2009 (diff | hist) N Template:Team:KULeuven/13 September 2009/VanillinReceptor (New page: * A was again ented for a pointmutation tomorrow * new ligation was done for X) (top)
- 12:55, 17 October 2009 (diff | hist) N Template:Team:KULeuven/11 September 2009/VanillinReceptor (New page: *Glycerolstock was made for virG and a sample was send for seqencing * virA was ented * new TOPOcloning was done for rpoA with a new protocol we recieved) (top)
- 12:53, 17 October 2009 (diff | hist) N Template:Team:KULeuven/10 September 2009/VanillinReceptor (New page: * puc+A was plated * X+K was again elctroporated) (top)
- 12:50, 17 October 2009 (diff | hist) N Template:Team:KULeuven/9 September 2009/VanillinReceptor (New page: * fluid cultures were made of rpoA, X+K and the motherplate of virG so we could miniprep this tomorrow *ligation of puc and A was electroporated * new ligation of X+K was tried out) (top)
- 12:47, 17 October 2009 (diff | hist) N Template:Team:KULeuven/8 September 2009/VanillinReceptor (New page: * restriction digest of A and puc were done * overnight ligation of puc and A) (top)
- 12:46, 17 October 2009 (diff | hist) N Template:Team:KULeuven/7 September 2009/VanillinReceptor (New page: * a new restrictiondigest was done for puc and A because we decided that earlier results were not good enough. * There was a gelextraction, nanodrop and ligation done overnight. * The col...) (top)
- 12:42, 17 October 2009 (diff | hist) N Template:Team:KULeuven/4 September 2009/VanillinReceptor (New page: * gelextraction and nanodrop for puc and A. The digest was done well. * mutagenesis was tried with colony pCR on position 428) (top)
- 12:38, 17 October 2009 (diff | hist) N Template:Team:KULeuven/3 September 2009/VanillinReceptor (New page: * The Y+K ligation was miniprepped and nanodropped to make later on a stock on glycerol * We decided to use for virA a puc19-vector in stead of TOPO, puc was digested with Xba1 and Ecor1 a...) (top)
- 12:33, 17 October 2009 (diff | hist) N Template:Team:KULeuven/2 September 2009/VanillinReceptor (New page: * there was made a controllgel of virA for our basicproduct, purification and nanodrop * the two ligations X+K were checked and electroporated * X was digested again and ligated in K * The...) (top)
- 12:28, 17 October 2009 (diff | hist) Template:Team:KULeuven/31 August 2009/VanillinReceptor (top)
- 12:27, 17 October 2009 (diff | hist) N Template:Team:KULeuven/1 September 2009/VanillinReceptor (New page: * There was a gel made to check if basicproduct W was correctly PCRed, and if the restriction digest of X,Y and K was good. * rpoA mutation on 202 was put on gel but there was no result * ...) (top)
- 12:21, 17 October 2009 (diff | hist) N Template:Team:KULeuven/31 August 2009/VanillinReceptor (New page: * TOPO cloning showed only blue colonies so we searched for alternatives. We did resaerchwork if it was maybe possible with a puc-vector * The mutation of rpoA was again checked but gelele...)
- 12:15, 17 October 2009 (diff | hist) N Template:Team:KULeuven/28 August 2009/VanillinReceptor (New page: * new TOPO-cloning was done but with a change in protocol. Ligationtime of the fragement in the vector was changed from 5 minutes to overnight. *mutagenesis of rpoA on 202 was done but th...) (top)
- 12:10, 17 October 2009 (diff | hist) Template:Team:KULeuven/27 August 2009/VanillinReceptor (top)
- 12:09, 17 October 2009 (diff | hist) N Template:Team:KULeuven/27 August 2009/VanillinReceptor (New page: * Because of bad results yesterday the transformation of W was extra checked by performing a new PCR with different primers : sample A : M13forward - virAforward sample B : M13forward - v...)
- 12:01, 17 October 2009 (diff | hist) Template:Team:KULeuven/26 August 2009/VanillinReceptor (top)
- 11:58, 17 October 2009 (diff | hist) N Template:Team:KULeuven/26 August 2009/VanillinReceptor (New page: - PCR for the second mutation of R was done - Electroporation of B and G after the ligation yesterday - PCR was done for controle of W to check if virA was inserted into the cell. The pict...)
- 14:05, 10 September 2009 (diff | hist) N Template:Team:KULeuven/25 August 2009/VanillinReceptor (New page: * PCR mutagenesis from R on 427, PCR purification and gelelectrophoresis : no signal on the the picture probably because enting didn't showed enough result * Ligation van X and Y in K14 at...)
- 13:45, 10 September 2009 (diff | hist) N Template:Team:KULeuven/24 August 2009/VanillinReceptor (New page: * TOPO vector with insert W showed some colonies. PCR + electrophoresis were performed for control but a bad and unclear picture was made so the result was not that visible * B and G vecto...)
- 13:00, 26 August 2009 (diff | hist) N Template:Team:KULeuven/21 August 2009/VanillinReceptor (New page: * TOPO cloning of W looks goods with clear white colonies. 5 colonies were selected and cultured for testing) (top)
- 12:53, 26 August 2009 (diff | hist) Template:Team:KULeuven/20 August 2009/VanillinReceptor
- 12:48, 26 August 2009 (diff | hist) N Template:Team:KULeuven/20 August 2009/VanillinReceptor (New page: * Continued with ethanol precipitation, electroporation and outplating (of R mutation 202))
- 12:46, 26 August 2009 (diff | hist) Template:Team:KULeuven/18 August 2009/VanillinReceptor
- 12:44, 26 August 2009 (diff | hist) Template:Team:KULeuven/17 August 2009/VanillinReceptor (top)
- 12:42, 26 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinReceptor
- 13:05, 25 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinReceptor
- 13:01, 25 August 2009 (diff | hist) N Template:Team:KULeuven/19 August 2009/VanillinReceptor (New page: * Continued with ethanol precipitation for R. Electroporation and outplating were performed) (top)
- 12:59, 25 August 2009 (diff | hist) N Template:Team:KULeuven/18 August 2009/VanillinReceptor (New page: * PCR purification miniprep and nanodrop * start ethanol precipitatie (overnight ethanol + sodium acetate) * PCR of the fragements of TOPO (W and Z) was done to check if TOPO worked. Gelel...)
- 12:54, 25 August 2009 (diff | hist) N Template:Team:KULeuven/17 August 2009/VanillinReceptor (New page: * A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out. * Fluid cul...)
- 12:47, 25 August 2009 (diff | hist) Template:Team:KULeuven/14 August 2009/VanillinReceptor
- 12:42, 25 August 2009 (diff | hist) Template:Team:KULeuven/13 August 2009/VanillinReceptor
- 12:40, 25 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinReceptor
- 12:39, 25 August 2009 (diff | hist) Template:Team:KULeuven/12 August 2009/VanillinReceptor
- 12:36, 25 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinReceptor
- 12:35, 25 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinReceptor
- 12:33, 25 August 2009 (diff | hist) Template:Team:KULeuven/11 August 2009/VanillinReceptor
- 09:57, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
- 09:56, 18 August 2009 (diff | hist) Team:KULeuven/Freezer (→vanillin receptor)
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