"
Uppsala-Sweden/25 August 2009
From 2009.igem.org
m |
|||
| (7 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
The plasmids holding our bio bricks were digested according to the file below. | The plasmids holding our bio bricks were digested according to the file below. | ||
| + | |||
[[Media:assembly_Plasmid_digestion.xls]] | [[Media:assembly_Plasmid_digestion.xls]] | ||
| + | |||
| + | The concentrationa measured after digestion and purification (NucleoSpin PCR clean up kit) | ||
| + | |||
| + | [[Media:digest_purification_measur_09825.txt]] | ||
| + | |||
| + | |||
| + | The volumes used for ligation is shown in the file below "digest_purification_measur_09825.htm" | ||
| + | |||
| + | [[Media:Ligation voulume calcuator_pSB1AK3_090825.xls]] | ||
| + | |||
This file "Biobricks and vectors" contains a list of ready bio bricked genes. | This file "Biobricks and vectors" contains a list of ready bio bricked genes. | ||
| + | |||
[[Media:Biobricks and vectors.xls]] | [[Media:Biobricks and vectors.xls]] | ||
| + | --[[User:Flormane|Flormane]] 18:16, 25 August 2009 (UTC) | ||
| + | |||
| + | ==Oligos for RNAi and Primers for PirA ordered== | ||
| + | |||
| + | [[media:Primer_Biolegio_20090825.xls]] | ||
| + | |||
| + | --[[User:Karl.brune|Karl.brune]] 18:37, 25 August 2009 (UTC) | ||
| + | |||
| + | ==Evaluating competence == | ||
| + | Three sets of cells were transformed with 1 l each pUC19 control plasmid. The cells were Hsin Ho's, our earlier and our new cells. The usual transformation procedure was followed.' | ||
| + | --[[User:Flormane|Flormane]] 13:09, 26 August 2009 (UTC) | ||
Latest revision as of 13:09, 26 August 2009
Assembly
The plasmids holding our bio bricks were digested according to the file below.
Media:assembly_Plasmid_digestion.xls
The concentrationa measured after digestion and purification (NucleoSpin PCR clean up kit)
Media:digest_purification_measur_09825.txt
The volumes used for ligation is shown in the file below "digest_purification_measur_09825.htm"
Media:Ligation voulume calcuator_pSB1AK3_090825.xls
This file "Biobricks and vectors" contains a list of ready bio bricked genes.
Media:Biobricks and vectors.xls --Flormane 18:16, 25 August 2009 (UTC)
Oligos for RNAi and Primers for PirA ordered
media:Primer_Biolegio_20090825.xls
--Karl.brune 18:37, 25 August 2009 (UTC)
Evaluating competence
Three sets of cells were transformed with 1 l each pUC19 control plasmid. The cells were Hsin Ho's, our earlier and our new cells. The usual transformation procedure was followed.' --Flormane 13:09, 26 August 2009 (UTC)
