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Template:Team:KULeuven/28 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
| pSb3K3 2 || 33,0 || 2,46 || | | pSb3K3 2 || 33,0 || 2,46 || | ||
|} | |} | ||
| - | # gel electroforesis of pSB3K3 miniprep and of restriction digest from 27/08 ({{kulpart|Bba_J23101}} and {{kulpart|Bba_E0240}} | + | # gel electroforesis of pSB3K3 miniprep and of restriction digest from 27/08 ({{kulpart|Bba_J23101}} and {{kulpart|Bba_E0240}}. There was an unexpected signal at 800bp at the ({{kulpart|Bba_J23101}} lanes. the conclusion was that this part can not be used and we have to start all over from the basic DNA to make this ligation C. |
| + | # we checked our cultures that were radiated with blue light on 27/08. | ||
| + | #*the liquid cultures were put in the FACs machine. there was some fluorecense which is probably due to GFP. however, it can not be excluded that this is due to leakage from our promotor. so a new set up needs to be made where decent controls are included. | ||
| + | #*the LB plates were put under a special GFP lamp. there was signal but the same considerations need to be made as with the liquid cultures. | ||
Revision as of 11:54, 28 August 2009
- miniprep of liquid cultures of pSB3K3 grown overnight.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| pSb3K3 1 | 36,4 | 2,23 | |
| pSb3K3 2 | 33,0 | 2,46 |
- gel electroforesis of pSB3K3 miniprep and of restriction digest from 27/08 ( and . There was an unexpected signal at 800bp at the ( lanes. the conclusion was that this part can not be used and we have to start all over from the basic DNA to make this ligation C.
- we checked our cultures that were radiated with blue light on 27/08.
- the liquid cultures were put in the FACs machine. there was some fluorecense which is probably due to GFP. however, it can not be excluded that this is due to leakage from our promotor. so a new set up needs to be made where decent controls are included.
- the LB plates were put under a special GFP lamp. there was signal but the same considerations need to be made as with the liquid cultures.
