Team:KU Seoul/Notebook

From 2009.igem.org

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Hi everyone. We narrowed our ideas to two subjects, which are "Morse Code" and "Bio Sticker." If you would like to share any other ideas, you are more then welcome. Let's discuss about specific models for these two subjects at the upcoming meeting.
Hi everyone. We narrowed our ideas to two subjects, which are "Morse Code" and "Bio Sticker." If you would like to share any other ideas, you are more then welcome. Let's discuss about specific models for these two subjects at the upcoming meeting.
-
Our next meeting is on 10th of July, 3 p.m. I recommend you think about at least one of the two subjects and how they should be developed. Tell me your schedules via mail so that I can fix our meeting time to the most suitable for all. I would like to share my experience at the iGEM workshop at Dongkyung about Wiki and other stuffs that you might b interested, so bring your notebooks.
+
Our next meeting is on 10th of July, 3 p.m. I recommend you think about at least one of the two subjects and how they should be developed. Tell me your schedules via mail so that I can fix our meeting time to the most suitable for all. I would like to share my experience at the iGEM workshop at Dongkyung about Wiki and other stuffs that you might be interested, so bring your notebooks.
As I said before, prepare a specific model for the meeting. Try to document your ideas so they could be shared with others. A specific model should include these:
As I said before, prepare a specific model for the meeting. Try to document your ideas so they could be shared with others. A specific model should include these:
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See you next Friday.
See you next Friday.
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'''From Cheolwon Choi, 5th of July'''
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This mail has a linked file that contains the lists of our team member's e-mails and contacts.
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'''From Cheolwon Choi, 10th of July'''
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Hi everyone. We need MORE ideas!!! We also need a clearer concept on synthetic biology. These are homeworks due to our next meeting, 17th of July.
 +
 +
1) https://2009.igem.org/Instructional_Videos Watch the videos.
 +
 +
2) Study the videos on iGEM 2009 home and tell us what you learned.
 +
 +
3) Divide our team into groups for each subject.
 +
 +
4) We need to learn more about Wiki. Wiki seems to take great part in judging. Although we are not that good in English, try to post your ideas. Try to visit our team Wiki everyday.
 +
 +
5) Please!!! Post me if you can't come.
 +
 +
 +
 +
'''From Simin Kim, 15th of July'''
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 +
Hey people. How's an aroma producing bacteria? If we can make a bacteria that can produce fragrance by using wasted food, we might not need to buy Febreeze anymore! :)
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 +
 +
'''From Cheolwon Choi, 19th of July'''
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 +
Gees. It poured cats and dogs during the weekends. Your ideas are due Monday. I appreciate your full participation.
 +
 +
 +
'''From Hansung, 19th of July'''
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 +
As I was searching the text books, I thought of making a "Bacteria Array," similar to DNA array. It can be built to sense specific substrates in a compound.
 +
 +
 +
'''From Kina Jeon, 20th of July'''
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 +
There is a molecule called Geosmin produced by microbes in water. It giveas the water a dirt flavor, an important factor for drinking water. We could build a bacteira that can sense geosmin and trap it, like the one Chiva university did on iGEM 2007.
 +
 +
 +
'''From Cheolwon Choi, 20th of July'''
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 +
If there is a way, I want to develop a way to increase the survival rates of diesel producing bacterias. This idea is also good for raising funds if we succeed! Can we also make bio gasolines?
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 +
 +
'''From Young Byun, 20th of July'''
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 +
Scrombroid food poisoning is the 2nd prevalent food posioning among fish and its main cause is histamine. Histidine which exists in all fish turns into histamine by histidine decarboxylase at temperature above 16 degrees celcius. Histmine is also noteworthy as a substrate for the expression of alergic reactions.
 +
 +
The basic mechanism is using "Band defect network" that was introduced in 'Towards Logical Designs in Biology.' GFP expression is repressed at low levels of histamine but strongly expressed at above threshold level. By this way, we can tell if the fish is edible or not. We are using a well known circuit here, so it might be a nice try.
 +
 +
I thought about the ideas our team produced. I think Bacteria Array is fascinating. But as for the aroma producing bacteria, can the small amount of fragrance produced overwhelm the stink of rotting food?
 +
 +
 +
'''From Cheolwon Choi, 21th of July'''
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 +
There are people still confused about how to use Google groups. It is extremely SIMPLE. Send your mail to synbiogroup@googlegroups.com. Yeap, that's all you need to do.
==Korean stuffs==
==Korean stuffs==
*[http://openwetware.org/wiki/IGEM:KU_Seoul/2009/Notebook/iGEM2009 Lab Notebook@Openwetware (한글)]
*[http://openwetware.org/wiki/IGEM:KU_Seoul/2009/Notebook/iGEM2009 Lab Notebook@Openwetware (한글)]

Revision as of 03:31, 29 August 2009

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Sponsors

Contents

Lab

Protocols (from elsewhere and FAQs)

  • How do I test the transformation efficiency of my competent cells?
    • Please see the protocol on how to [http://www.openwetware.org/wiki/TOP10_chemically_competent_cells make competent cells] for instructions on how to calculate transformation efficiency.

Discussion

Team building

From instructors

  • 10.07.09 We had a review on our two ideas from Prof.Choi. He told us the ideas are creative and good but not fit for IGEM's concept. We need more feedback and information on our ideas. We need to get more familiar with IGEM's tools. Two additional ideas about Scromboid food poisoning and lactic acid degradation were referred at the end.
  • 17.07.09 Till our next meeting, we should study how systemic biology works and understand related tools. Cumulate ideas. In other words, brainstorm consistently. Hyuk-jin will help with setting up experiments. Lab note opend.
  • 24.07.09 We narrowed our ideas in to two. They are "Metal Detector" and "Bactaeria Compass." Think about what parts would be used and how you would build the circuits. Visit the former iGEM teams and study how they carried on their projects.
  • 31.07.09 Find out the exact mechanism of metal detection. Look up the parts that are available on iGEM home. See how various metals can be detected by using E.coli.
  • 07.08.09 We decided to detect 4 metals; Cd, Hg, Pb, and Ars. They are all critical to the health if ingested. Think about what the outcome would be after detection. Because there are 4 metals, you can bring out 16 different results by combining them. It seems that the most vialbe instrument would be light.

Brainstroming

Ideas

- Biosurfactants

- Keratinase

- Deodorant

- Perfume-producing bacteria

- Heat absorber

- Bio pencil

Should we focus on the result, or the procedure?

Decision making

From Cheolwon Choi, 4th of July

Hi everyone. We narrowed our ideas to two subjects, which are "Morse Code" and "Bio Sticker." If you would like to share any other ideas, you are more then welcome. Let's discuss about specific models for these two subjects at the upcoming meeting. Our next meeting is on 10th of July, 3 p.m. I recommend you think about at least one of the two subjects and how they should be developed. Tell me your schedules via mail so that I can fix our meeting time to the most suitable for all. I would like to share my experience at the iGEM workshop at Dongkyung about Wiki and other stuffs that you might be interested, so bring your notebooks. As I said before, prepare a specific model for the meeting. Try to document your ideas so they could be shared with others. A specific model should include these:

1. How will the chosen subject be used?

2. What are its limits?

3. What materials would be needed?

4. What former knowledge would be required to process the subject?

5. Why did you choose the subject?

6. What contributions can you make?

7. Etc.

See you next Friday.


From Cheolwon Choi, 5th of July

This mail has a linked file that contains the lists of our team member's e-mails and contacts.


From Cheolwon Choi, 10th of July

Hi everyone. We need MORE ideas!!! We also need a clearer concept on synthetic biology. These are homeworks due to our next meeting, 17th of July.

1) https://2009.igem.org/Instructional_Videos Watch the videos.

2) Study the videos on iGEM 2009 home and tell us what you learned.

3) Divide our team into groups for each subject.

4) We need to learn more about Wiki. Wiki seems to take great part in judging. Although we are not that good in English, try to post your ideas. Try to visit our team Wiki everyday.

5) Please!!! Post me if you can't come.


From Simin Kim, 15th of July

Hey people. How's an aroma producing bacteria? If we can make a bacteria that can produce fragrance by using wasted food, we might not need to buy Febreeze anymore! :)


From Cheolwon Choi, 19th of July

Gees. It poured cats and dogs during the weekends. Your ideas are due Monday. I appreciate your full participation.


From Hansung, 19th of July

As I was searching the text books, I thought of making a "Bacteria Array," similar to DNA array. It can be built to sense specific substrates in a compound.


From Kina Jeon, 20th of July

There is a molecule called Geosmin produced by microbes in water. It giveas the water a dirt flavor, an important factor for drinking water. We could build a bacteira that can sense geosmin and trap it, like the one Chiva university did on iGEM 2007.


From Cheolwon Choi, 20th of July

If there is a way, I want to develop a way to increase the survival rates of diesel producing bacterias. This idea is also good for raising funds if we succeed! Can we also make bio gasolines?


From Young Byun, 20th of July

Scrombroid food poisoning is the 2nd prevalent food posioning among fish and its main cause is histamine. Histidine which exists in all fish turns into histamine by histidine decarboxylase at temperature above 16 degrees celcius. Histmine is also noteworthy as a substrate for the expression of alergic reactions.

The basic mechanism is using "Band defect network" that was introduced in 'Towards Logical Designs in Biology.' GFP expression is repressed at low levels of histamine but strongly expressed at above threshold level. By this way, we can tell if the fish is edible or not. We are using a well known circuit here, so it might be a nice try.

I thought about the ideas our team produced. I think Bacteria Array is fascinating. But as for the aroma producing bacteria, can the small amount of fragrance produced overwhelm the stink of rotting food?


From Cheolwon Choi, 21th of July

There are people still confused about how to use Google groups. It is extremely SIMPLE. Send your mail to synbiogroup@googlegroups.com. Yeap, that's all you need to do.

Korean stuffs

  • [http://openwetware.org/wiki/IGEM:KU_Seoul/2009/Notebook/iGEM2009 Lab Notebook@Openwetware (한글)]
Retrieved from "http://2009.igem.org/Team:KU_Seoul/Notebook"