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Template:Team:KULeuven/31 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
| {{kulpart|BBa_E0240}} || 104,3 || 2,10 || | | {{kulpart|BBa_E0240}} || 104,3 || 2,10 || | ||
|} | |} | ||
| - | + | * RD results on gel: the right signals were detected | |
| - | #ligA was plated on LB medium | + | # ligA was plated on LB medium. this will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from. |
| - | #electroporation of J23101 | + | # electroporation of J23101 in competent cells. it was plated and put overnight in the incubator. |
| + | # in order to be able to grow vector pSB3K3, which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. this is strain DB3.1 from Ecoli. these were plated and put in the incubator overnight. | ||
Revision as of 19:23, 31 August 2009
- a Miniprep and RD (with EcoRI and PstI) was performed on the four colonies that were ented on sunday.
- nanoprop results
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA (1) | 110,6 | 1,92 | |
| LigA (2) | 76,7 | 1,99 | |
| LigA (3) | 84,8 | 2,07 | |
| LigA (4) | 31,7 | 2,02 | |
| 104,3 | 2,10 |
- RD results on gel: the right signals were detected
- ligA was plated on LB medium. this will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from.
- electroporation of J23101 in competent cells. it was plated and put overnight in the incubator.
- in order to be able to grow vector pSB3K3, which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. this is strain DB3.1 from Ecoli. these were plated and put in the incubator overnight.
