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Template:Team:KULeuven/31 August 2009/BlueLightReceptor
From 2009.igem.org
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# ligA was plated on LB medium. this will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from. | # ligA was plated on LB medium. this will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from. | ||
| - | # electroporation of | + | # electroporation of {{kulpart|BBa_J23101}} in competent cells. it was plated and put overnight in the incubator. |
| - | # in order to be able to grow vector pSB3K3, which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. this is strain DB3.1 from Ecoli. these were plated and put in the incubator overnight. | + | # in order to be able to grow vector {{kulpart|pSB3K3}}, which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. this is strain DB3.1 from Ecoli. these were plated and put in the incubator overnight. |
Revision as of 12:00, 1 September 2009
- a Miniprep and RD (with EcoRI and PstI) were performed on the four colonies that were ented on sunday.
- nanoprop results
- RD results on gel: the right signals were detected
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| LigA (1) | 110,6 | 1,92 | |
| LigA (2) | 76,7 | 1,99 | |
| LigA (3) | 84,8 | 2,07 | |
| LigA (4) | 31,7 | 2,02 | |
| 104,3 | 2,10 |
- ligA was plated on LB medium. this will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from.
- electroporation of in competent cells. it was plated and put overnight in the incubator.
- in order to be able to grow vector , which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. this is strain DB3.1 from Ecoli. these were plated and put in the incubator overnight.
