Template:Team:KULeuven/20 August 2009/VanillinReceptor

From 2009.igem.org

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* Restriction digest of X, Y and K145201 with EcoR1 and Pst1
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* Restriction digest of X, Y and {{kulpart|BBa_K145201}} with EcoR1 and Pst1
* Gelelectroforesis to check fragments and gelextraction
* Gelelectroforesis to check fragments and gelextraction
* Fluid medium from outplated cells (overgrowth)
* Fluid medium from outplated cells (overgrowth)
* TOPO-cloning (with new protocol from invitrogen) for W and Z
* TOPO-cloning (with new protocol from invitrogen) for W and Z

Revision as of 12:28, 1 September 2009

  • Restriction digest of X, Y and with EcoR1 and Pst1
  • Gelelectroforesis to check fragments and gelextraction
  • Fluid medium from outplated cells (overgrowth)
  • TOPO-cloning (with new protocol from invitrogen) for W and Z